Osong Public Health Res Perspect.  2022 Aug;13(4):290-297. 10.24171/j.phrp.2022.0150.

The effect of photodynamic therapy using Radachlorin on biofilm-forming multidrug-resistant bacteria

Affiliations
  • 1Department of Biomedical Laboratory Science, Dongeui Institute of Technology, Busan, Korea
  • 2Department of Health Sciences, The Graduate School of Konyang University, Daejeon, Korea
  • 3Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan, Korea
  • 4Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University, Daejeon, Korea

Abstract


Objectives
This study aimed to test the effect of photodynamic therapy (PDT) on the inhibition and removal of biofilms containing multidrug-resistant Acinetobacter baumannii.
Methods
Using multidrug-resistant A. baumannii strains, an antibiotic susceptibility test was performed using the Gram-negative identification card of the Vitek 2 system (bioMérieux Inc., France), as well as an analysis of resistance genes, the effects of treatment with a light-emitting diode (LED) array using Radachlorin (RADA-PHARMA Co., Ltd., Russia), and transmission and scanning electron microscopy to confirm the biofilm-inhibitory effect of PDT.
Results
The antibiotic susceptibility test revealed multiple resistance to the antibiotics imipenem and meropenem in the carbapenem class. A class-D–type β-lactamase was found, and OXA-23 and OXA-51 were found in 100% of 15 A. baumannii strains. After PDT using Radachlorin, morphological observations revealed an abnormal structure due to the loss of the cell membrane and extensive morphological changes, including low intracellular visibility and small vacuoles attached to the cell membrane.
Conclusion
PDT involving a combination of LED and Radachlorin significantly eliminated the biofilm of multidrug-resistant A. baumannii. Observations made using electron microscopy showed that PDT combining LED and Radachlorin was effective. Additional studies on the effective elimination of biofilms containing multidrug-resistant bacteria are necessary, and we hope that a treatment method superior to sterilization with antibiotics will be developed in the future.

Keyword

Acinetobacter baumannii; Light-emitting diode; Photochemotherapy; Radachlorin
Full Text Links
  • OPHRP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr