Ann Clin Microbiol.  2022 Jun;25(2):59-65. 10.5145/ACM.2022.25.2.4.

Current Prevalence of the crpP Gene in Carbapenemase-Producing Pseudomonas aeruginosa Blood Isolates in Korea

• Heo J
• Choi YJ
• Kim YA
• Jeong SH
• Shin JH
• Shin KS
• Shin JH
• Kim YR
• Kim HS
• Uh Y
• Ryoo NH

• KMID: 2530722
• DOI: http://doi.org/10.5145/ACM.2022.25.2.4

Abstract

Background
Recently, CrpP enzymes have been described as a novel cause of ciprofloxacin resistance. The crpP gene encodes a novel protein that specifically confers resistance to ciprofloxacin through an adenosine triphosphate-dependent mechanism that phosphorylates the antimicrobial. In this study, the current prevalence of the crpP gene in carbapenemaseproducing Pseudomonas aeruginosa blood isolates was evaluated.
Methods
During the study of the Antimicrobial Resistance Surveillance System in Korea, 22 blood isolates of carbapenemase-producing P. aeruginosa were collected from nine general hospitals and two nursing homes in the year 2020. Resistance genes and phylogenic trees were analyzed with the whole genome sequencing data.
Results
A total of 11 P. aeruginosa blood isolates coharbored the crpP and carbapenemase genes (nine IMP-6 producers and two GES-5-producers). Nine NDM-1-producers coharbored aac(6')-Ib-cr and qnrVC1 . One GES-9-producer also carried aac(6')-Ib-cr, and one NDM-1-producer also carried qnrVC1. The phylogenic tree showed no epidemiologic link among the 22 carbapenemase-producing P. aeruginosa isolates.
Conclusion
This is the first report on the current prevalence of the crpP gene in carbapenemaseproducing P. aeruginosa blood isolates in Korea.

Keyword

Fluoroquinolone; Resistance; crpP gene; Pseudomonas aeruginosa