Oral Biol Res.  2021 Sep;45(3):115-118. 10.21851/obr.45.03.202109.115.

Comparison between the Rapid ID 32 Strep test system and 16S rRNA gene sequencing for the identification of α-hemolytic streptococci

• Lee SY

• KMID: 2521023
• DOI: http://doi.org/10.21851/obr.45.03.202109.115

Abstract

Oral viridans streptococcus is the most common bacterial species present in human dental plaque. The Rapid ID 32 Strep (bioMerieux, Marcy-l‘Etoile, France) test system allows for the identification of most streptococci within 4 hours. The aim of this study was to evaluate the reliability of this test in identifying isolates of α-hemolytic oral streptococci in human dental plaque and compare it with that of the 16S rRNA sequencing method. A total of eighty-five α-hemolytic streptococcal isolates were identified using 16S rRNA gene sequencing and Rapid ID 32 Strep. The Rapid ID 32 Strep method correctly identified 80% of the species and 87% of the streptococcal groups but failed to accurately identify five isolates (three S. cristatus, one S. australis, and one S. tigurinus).

Keyword

Identification; Sequencing; Streptococci