Immune Netw.  2021 Jun;21(3):e24. 10.4110/in.2021.21.e24.

Effect of Human or Mouse IL-7 on the Homeostasis of Porcine T Lymphocytes

Affiliations
  • 1Departments of Pharmacy, Korea University, Sejong 30019, Korea
  • 2Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Korea
  • 3Futuristic Animal Resource & Research Center (FARRC), Cheongju 28116, Korea
  • 4National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Korea
  • 5Primate Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56216, Korea

Abstract

Due to the inconsistent fluctuation of blood supply for transfusion, much attention has been paid to the development of artificial blood using other animals. Although mini-pigs are candidate animals, contamination of mini-pig T cells in artificial blood may cause a major safety concern. Therefore, it is important to analyze the cross-reactivity of IL-7, the major survival factor for T lymphocytes, between human, mouse, and mini-pig. Thus, we compared the protein sequences of IL-7 and found that porcine IL-7 was evolutionarily different from human IL-7. We also observed that when porcine T cells were cultured with either human or mouse IL-7, these cells did not increase the survival or proliferation compared to negative controls. These results suggest that porcine T cells do not recognize human or mouse IL-7 as their survival factor.

Keyword

IL-7; Miniature swine; Graft-versus-host disease; T-lymphocytes
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