Ann Dermatol.  2021 Aug;33(4):318-323. 10.5021/ad.2021.33.4.318.

Optimal Fluence and Duration of Low-Level Laser Therapy for Efficient Wound Healing in Mice

• Yoon J
• Park JH
• Choi JW
• Kim YC

• KMID: 2517837
• DOI: http://doi.org/10.5021/ad.2021.33.4.318

Abstract

Background
Low-level laser (light) therapy is a promising technology that stimulates healing, relieves pain and inflammation, and restores function in injured body parts. However, few studies have compared the effects of lightemitting diodes of different fluence levels or different treatment durations.
Objective
Here, we investigated the effects of various fluence levels and treatment durations on wound closure in mice.
Methods
Full-thickness wounds were created on the dorsal skin using an 8-mm diameter punch, and the wounds were irradiated at 1, 4, or 40 J/cm 2 for 5 consecutive days starting on day 1. To determine the optimal irradiation duration, wounds were irradiated at the most potent fluence of previous study for 5, 10, or 15 days. Photographic documentation, skin biopsies, and wound measurements were performed to compare the effects of different treatment parameters.
Results
The most effective fluence level was 40 J/cm 2 at day 5, as determined by monitoring wound closure. There were no statistically significant differences in wound healing with different durations.
Conclusion
We have shown that repeated exposure to low levels of light significantly stimulates wound healing in mice and demonstrated more efficient wound closure with certain fluences of 830 nm irradiation.

Keyword

Laser; Low-level light therapy; Wound healing