Restor Dent Endod.  2020 Aug;45(3):e40. 10.5395/rde.2020.45.e40.

Effects of endodontic treatment on salivary levels of CGRP and substance P: a pilot study

• Arslan H
• Yıldız ED
• Köseoğlu S

• KMID: 2512031
• DOI: http://doi.org/10.5395/rde.2020.45.e40

Abstract

Objectives
The aim of this study was to evaluate the effects of endodontic treatment on levels of substance P (SP) and calcitonin gene-related peptide (CGRP) in the saliva of patients with symptomatic apical periodontitis.
Materials and Methods
Twelve patients with mandibular molars with symptomatic apical periodontitis were enrolled in this study. An initial saliva sample was collected just before administration of anesthesia for root canal treatment, which was performed at the first visit. A second saliva sample was collected at a control visit 1 week after treatment. Salivary SP and CGRP levels were evaluated quantitatively using biochemical assays. The data were analyzed using Pearson correlation analysis, the paired samples t-test, and the Mann-Whitney U test (p = 0.05).
Results
The postoperative salivary level of SP was significantly lower than the preoperative level (p = 0.005). However, the postoperative salivary level of CGRP was similar to the preoperative level (p = 0.932). Visual analog scale (VAS) scores of patients' subjective pain were found to be positively correlated with salivary levels of SP (r = 0.421; p = 0.040). No statistically significant correlations were observed between salivary levels of CGRP and VAS scores for patients' subjective percussion tenderness (p = 0.533) or VAS scores for patients' subjective pain (p = 0.459).
Conclusions
According to the results of the present study, salivary SP levels may be used as an objective indicator in the diagnosis and assessment of the degree of pain in endodontic diseases.

Keyword

Endodontics; Substance P; Calcitonin gene-related peptide; Root canal treatment

Figure

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  Figure 1 Flow diagram.

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