Korean J Dent Mater.  2020 Dec;47(4):245-252. 10.14815/kjdm.2020.47.4.245.

Sustained antibacterial effects of antibacterial agents against Streptococcus mutans applied on hydroxyapatite disc

  • 1Department of Dental Hygiene, Wonkwang Health Science University, Iksan-si, Republic of Korea
  • 2College of Dentistry, Wonkwang University, IIksan-si, Republic of Korea
  • 3Department of Dental Biomaterials and Institute of Biomaterials·Implant, College of Dentistry, Wonkwang University, Iksan-si, Republic of Korea


This study aimed to evaluate the antibacterial activities sustainability of antibacterial agents mixed with experimental fluoride varnish on hydroxyapatite disc against Streptococcus mutans (S. mutans). On the hydroxyapatite disc with 4.8 mm of diameter, 5 μL of 1 mM antibacterial agents mixed with experimental fluoride varnish were applied. Positive control and vehicle control were 10 μg/mL ampicillin and dimethyl sulfoxide (DMSO) mixed with fluoride varnish, respectively. The discs were stored in distilled water in a 37℃ shaking water bath at 120 rpm for 30 minutes, 4 hours, 1 day, 5 days, 20 days, and 30 days. Antibacterial activities were evaluated with the inhibition zone by the agar diffusion test. The antibacterial activities of all antibacterial agents were sustained for 30 days. Among them, Bakuchiol, Bavachromene, and Bavachalcone showed higher antibacterial activities for up to 30 days. The antibacterial agents when mixed in the experimental fluoride varnish with increased and prolonged antibacterial activities can be applied to prevent dental caries effectively.


Sustained antibacterial effect; Fluoride varnish; Antibacterial agents; Streptococcus mutans
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