Immune Netw.
2020 Oct;20(5):e41. 10.4110/in.2020.20.e41.
The Progression of SARS Coronavirus 2 (SARS-CoV2): Mutation in the Receptor Binding Domain of Spike Gene
- Kim S
1,2 - Lee JH3
- Lee S1,4
- Shim S1
- Nguyen TT1,2
- Hwang J1
- Kim H1
- Choi YO1
- Hong J1,2
- Bae S1,5
- Jhun H1,6
- Yum H7
- Lee Y8
- Chan ED9
- Yu L10
- Azam T11
- Kim YD12
- Yeom SC13
- Yoo KH14
- Kang LW15
- Shin KC16
- Kim S1,2,17
- Affiliations
-
- 1Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, Seoul 05029, Korea
- 2College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea
- 3Department of Laboratory Medicine, College of Medicine, Yeungnam University, Daegu 42415, Korea
- 4YbdYbiotech Research Center, Seoul 08589, Korea
- 5Cardiovascvular and Neuropharmacological Drugs Division, Drug Evaluation Department, Ministry of Food and Drug Safety, Cheongju 28159, Korea
- 6Technical Assistance Center, Korea Food Research Institute, Wanju 55365, Korea
- 7Pulmonary Science and Critical Care Medicine, Seoul Paik Hospital, Inje University, Seoul 04551, Korea
- 8Department of Medicine, Pusan Paik Hospital, Collage of Medicine, Inje University, Busan 47392, Korea
- 9Division of Pulmonary, Critical Care and Sleep Medicine, National Jewish Health, Denver, CO 80206, USA
- 10Barbara Davis Center for Diabetes, University of Colorado Denver, Aurora, CO 80045, USA
- 11Department of Medicine, University of Colorado Denver, Aurora, CO 80045, USA
- 12Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu 42415, Korea
- 13Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea
- 14Division of Pulmonology, Department of Internal Medicine, School of Medicine, Konkuk University, Seoul 05029, Korea
- 15Department of Biological Sciences, Konkuk University, Seoul 05029, Korea
- 16Center for Respiratory Disease, Yeungnam University, Daegu 42415, Korea
- 17Veterinary Science Research Institute, Konkuk University, Seoul 05029, Korea
- KMID: 2511866
- DOI: http://doi.org/10.4110/in.2020.20.e41
Abstract
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) is a positive-sense singlestranded RNA (+ssRNA) that causes coronavirus disease 2019 (COVID-19). The viral genome encodes twelve genes for viral replication and infection. The third open reading frame is the spike (S) gene that encodes for the spike glycoprotein interacting with specific cell surface receptor – angiotensin converting enzyme 2 (ACE2) – on the host cell membrane. Most recent studies identified a single point mutation in S gene. A single point mutation in S gene leading to an amino acid substitution at codon 614 from an aspartic acid 614 into glycine (D614G) resulted in greater infectivity compared to the wild type SARS-CoV2. We were interested in investigating the mutation region of S gene of SARS-CoV2 from Korean COVID-19 patients. New mutation sites were found in the critical receptor binding domain (RBD) of S gene, which is adjacent to the aforementioned D614G mutation residue. This specific sequence data demonstrated the active progression of SARS-CoV2 by mutations in the RBD of S gene. The sequence information of new mutations is critical to the development of recombinant SARS-CoV2 spike antigens, which may be required to improve and advance the strategy against a wide range of possible SARS-CoV2 mutations.
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