Nat Prod Sci.  2020 Jun;26(2):158-164. 10.20307/nps.2020.26.2.158.

Anti-Helicobacter pylori Activity of Compounds Isolated from Fraxinus mandshurica Bark

Affiliations
  • 1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Korea
  • 2Department of Biological Sciences, National University of Medical Sciences, 46000 Rawalpindi, Pakistan

Abstract

Helicobacter pylori is a well-known pathogen that is responsible for gastric disorders. Overcoming of the antibiotic-resistance is a main barrier to treat H. pylori infection. In our search for anti-H. pylori compounds from natural resources, bioactivity-guided isolation on the ethyl acetate fraction of Fraxinus mandshurica bark that had shown anti-H. pylori activity gave twelve compounds (1 - 12) of six coumarins, three phenylethanoids, two secoiridoids, and a lignan using silica gel column chromatography, Sephadex-LH 20, and recrystallization. The chemical structures were identified by spectroscopic data analysis, including 1D, 2D NMR, and mass spectrometry. Among them, compounds 2, 10, and 11 showed moderate growth inhibitory activity against three strains ofH. pylori, compared with positive controls of quercetin and metronidazole. Compounds 5, 6, 8, and 12 exhibited the inhibitory activity against strains 26695 or 43504. This is the first report on the anti-H. pylori activity of this plant and the isolated compounds.

Keyword

Fraxinus mandshurica; Anti-Helicobacter pylori; Fraxetin; Calceolarioside A; Calceolarioside B
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