Int J Oral Biol.  2020 Sep;45(3):77-83. 10.11620/IJOB.2020.45.3.77.

Practical considerations for the study of the oral microbiome

Affiliations
  • 1Interdisplinary Program of Genomic Science, Pusan National University, Yangsan 50612, Republic of Korea
  • 2Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea

Abstract

In the oral cavity, complex microbial community is shaped by various host and environmental factors. Extensive literature describing the oral microbiome in the context of oral health and disease is available. Advances in DNA sequencing technologies and data analysis have drastically improved the analysis of the oral microbiome. For microbiome study, bacterial 16S ribosomal RNA gene amplification and sequencing is often employed owing to the cost-effective and fast nature of the method. In this review, practical considerations for performing a microbiome study, including experimental design, molecular analysis technology, and general data analysis, will be discussed.

Keyword

Oral microbiome; 16S rDNA; Bacterial associations; High-throughput nucleotide sequencing
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