J Vet Sci.  2020 Mar;21(2):e19. 10.4142/jvs.2020.21.e19.

Characterization of Newcastle disease virus obtained from toco toucan

• Li J
• Ling M
• Sun Y
• Di H
• Cong Y
• Yu H
• Cong Y

• KMID: 2506180
• DOI: http://doi.org/10.4142/jvs.2020.21.e19

Abstract

Given that the current Newcastle disease virus (NDV) infection in wild birds poses the threat to poultry, surveillance of Newcastle disease in captive wild birds was carried out in Jilin, China in 2018. Here, an NDV strain obtained from toco toucan was firstly characterized. The results showed that the F gene of the NDV isolate Toucan/China/3/2018 is classified as genotype II in class II. Sequence analysis of the F0 cleavage site was 113 RQGR/L 117 , which supports the result of the intracerebral pathogenicity index assay indicating classification of the isolate as low-pathogenicity. Experimental infection demonstrated that Toucan/ China/3/2018 can effectively replicate and transmit among chickens. To our knowledge, this is the first report on genetically and pathogenically characterizing NDV strain isolated from toucan, which enriches the epidemiological information of NDV in wild birds.

Keyword

Toucan; NDV; genetic analysis; transmission potential

Figure

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  Fig. 1. Phylogenetic analysis based on the open reading frame (1–1,662 nt) of F genes from 20 genotypes of Newcastle disease virus. The tree was created by the maximum-likelihood method and bootstrapped with 1,000 replicates. Toucan isolate analyzed in the present study is in italics.

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  Fig. 2. Phylogenetic analysis based on the full-length (1–1,662 nt) sequence of F genes from genotype II of Newcastle disease virus available in GenBank. The tree was created by the maximum-likelihood method and bootstrapped with 1,000 replicates. Only bootstrap values above 50 are shown. The vaccine strains are shown as solid black circles. Toucan strain analyzed in the present study is shown as a solid black triangle.

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  Fig. 3. Immunohistochemistry analysis of tissue samples from chickens intranasally inoculated with Toucan/China/3/2018 at 7 days post inoculation. Arrows indicate the positive labeling of Newcastle disease virus. (A) Brain; (B) thymus; (C) trachea; (D) lung; (E) heart; (F) liver; (G) spleen; (H) glandular stomach; (I) small intestine; (J) pancreas; (K) kidney; and (L) bursa of Fabricius. Arrows indicate magnification, 20×.

Reference

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