Mycobiology.  2020 Jun;48(3):219-227. 10.1080/12298093.2020.1746064.

The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells

Affiliations
  • 1Division of Urology, Department of Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan City, Taiwan
  • 2Institute of Biotechnology, National Taiwan University, Taipei, Taiwan
  • 3Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
  • 4Department of Internal Medicine, Taipei Medical University–Shuang Ho Hospital, New Taipei City, Taiwan
  • 5Graduate Institute of Food Science Technology, National Taiwan University, Taipei, Taiwan
  • 6Department of Medical Research, China Medical University Hospital, China. Medical University, Taichung, Taiwan

Abstract

Androgen-independent prostate cancer accounts for mortality in the world. In this study, various extracts of a medical fungus dubbed Ganoderma formosanum were screened for inhibition of DU145 cells, an androgen-independent prostate cancer cell line. Results demonstrated that both hexane (GF-EH) and butanol (GF-EB) fraction of G. formosanum ethanol extract inhibited DU145 cell viability in a dose-dependent manner. GF-EH induced cell-cycle arrest in G1 phase of DU145 cells via downregulation of cyclin E2 protein expression. In addition, GF-EB triggered extrinsic apoptosis of DU145 cells by activating caspase 3 gene expression resulting in programed cell death. Above all, both GF-EH and GF-EB show lower toxicity to normal human fibroblast cell line compared to DU145 cell, implying that they possess specific drug action on cancer cells. This study provides a molecular basis of G. formosanum extract as a potential ingredient for treatment of androgen-independent prostate cancer.

Keyword

Prostate cancer; Ganoderma formosanum; cell cycle arrest; cyclin; apoptosis
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