Mycobiology.  2020 Apr;48(2):153-156. 10.1080/12298093.2020.1732008.

Cumulative Effects of Constituents from the Mushroom Calvatia nipponica on the Contractility of Penile Corpus Cavernosum Smooth Muscle

  • 1School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea
  • 2College of Pharmacy, Kyungsung University, Busan, Republic of Korea
  • 3Special Forest Products Division, Forest Bioresources Department, National Institute of Forest Science, Suwon, Republic of Korea


Calvatia nipponica, a puffball mushroom (Agaricaceae), is thought to be an aphrodisiac, as this mushroom is traditionally known to improve sexual function in males. As part of the systematic study to determine the bioactive secondary metabolites from C. nipponica responsible for aphrodisiac effects, chemical analysis of methanol (MeOH) extracts of the fruiting bodies of C. nipponica resulted in the isolation of two major compounds: N,Ndimethyl- anthranilic acid (1) and (7Z,10Z)-7,10-octadecadienoic acid methyl ester (2). Compounds 1 and 2 were evaluated for cumulative dose-dependent relaxation responses to precontracted penile corpus smooth muscle (PCCSM). Results show that compounds 1 and 2 exhibited a maximum relaxation effect of 20.33 ± 2.18% and 24.63 ± 3.60%, respectively. These findings indicate that compounds 1 and 2, major components of C. nipponica, could potentially be used to treat erectile dysfunction, functioning as natural aphrodisiacs.


Calvatia nipponica; puffball mushroom; N,N-dimethylanthranilic acid; (7Z,10Z)- 7,10-octadecadienoic acid methyl ester; precontracted penile corpus smooth muscle (PCCSM)
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