Ann Lab Med.  2020 Sep;40(5):424-427. 10.3343/alm.2020.40.5.424.

False Positive Class II HLA Antibody Reaction Due to Antibodies Against Denatured HLA Might Differ Between Assays: One Lambda vs. Immucor

Affiliations
  • 1Department of Laboratory Medicine, Korea University Guro Hospital, Seoul, Korea
  • 2Departments of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea
  • 3Departments of Nephrology, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea
  • 4Departments of Transplant Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea


Figure

  • Fig. 1 Reactivity patterns of class II HLA antibody assays. (A) LABScreen SAB class II (One Lambda, Canoga Park, LA, USA) SABA showing pan-HLA-DR positivity. Self-HLA-DR antigens are indicated by red circles. (B) Lifecodes class II SABA (Immucor, Stamford, CT, USA) showing positivity for HLA-DR51 and -DR9. (C) PRA Identification Bead Reaction (LABScreen PRA, One Lambda) showing weak positivity for HLA-DR51, DR15, and DR16. Abbreviations: see Table 1.


Reference

1. Lobashevsky AL. Methodological aspects of anti-human leukocyte antigen antibody analysis in solid organ transplantation. World J Transplant. 2014; 4:153–67.
Article
2. El-Awar N, Terasaki PI, Nguyen A, Sasaki N, Morales-Buenrostro LE, Saji H, et al. Epitopes of human leukocyte antigen class I antibodies found in sera of normal healthy males and cord blood. Hum Immunol. 2009; 70:844–53.
Article
3. Visentin J, Guidicelli G, Bachelet T, Jacquelinet C, Audry B, Nong T, et al. Denatured class I human leukocyte antigen antibodies in sensitized kidney recipients: prevalence, relevance, and impact on organ allocation. Transplantation. 2014; 98:738–44.
4. Morales-Buenrostro LE, Terasaki PI, Marino-Vazquez LA, Lee JH, El-Awar N, Alberu J. “Natural” human leukocyte antigen antibodies found in nonalloimmunized healthy males. Transplantation. 2008; 86:1111–5.
Article
5. In JW, Rho EY, Shin S, Park KU, Song EY. False-positive reactions against HLA class II molecules detected in Luminex single-antigen bead assays. Ann Lab Med. 2014; 34:408–10.
Article
6. Grenzi PC, de Marco R, Silva RZR, Campos EF, Gerbase-DeLima M. Antibodies against denatured HLA class II molecules detected in Luminex-single antigen assay. Hum Immunol. 2013; 74:1300–3.
Article
7. Roufosse C, Simmonds N, Groningen MC, Haas M, Henriksen KJ, Horsfield C, et al. A 2018 reference guide to the Banff classification of renal allograft pathology. Transplantation. 2018; 102:1795–814.
Article
8. Visentin J, Marroc M, Guidicelli G, Bachelet T, Nong T, Moreau JF, et al. Clinical impact of preformed donor-specific denatured class I HLA antibodies after kidney transplantation. Clin Transplant. 2015; 29:393–402.
Article
9. El-Awar N, Lee JH, Tarsitani C, Terasaki PI. HLA class I epitopes: recognition of binding sites by mAbs or eluted alloantibody confirmed with single recombinant antigens. Hum Immunol. 2007; 68:170–80.
Article
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