J Korean Orthop Assoc.  1975 Jun;10(2):149-155. 10.4055/jkoa.1975.10.2.149.

In Vitro Observation of Phagocytic Activities of Normal Rabbit Macrophages on Ps. Aeruginosa and B. Subtilis

• Kim CK

• KMID: 2471387
• DOI: http://doi.org/10.4055/jkoa.1975.10.2.149

Abstract

Bacterial suspensions of Ps. aeruginosa and B. subtilis cultivated in Brain-Heart Infusion Broth and these mixture were added to the suspended rabbit macrophages in the serum of normal rabbit and Cell-Maintenance Fluid (CMF). After an incubation period of 30 and 60 minutes at 37℃, the bacteria-macrophage suspensions were investigated as regard to the percentage of macrophages with intracellular organisms and the percentage of intracellular organisms. Result of observation were summarized as follows: 1. In the Ps. aeruginosa-macrophage suspension; The percentages of macrophages with intracellular organisms were 15% after 30 minutes, 29% after 60 minutes in normal rabbit serum, and 11.5% and 46% after 30 and 60 minutes in CMF respectively. The percentages of intracellular organisms in normal rabbit serum were 97% after 30 and 60 minutes, and 92% after 30 minutes. 96% after 60 minutes in CMF. 2. In the B. subtilis-macrophage suspension; The percentages of macrophages with intracellular organisms in normal rabbit serum were 16% after 30 minutes, 26% after 60 minutes, and 27% after 30 minutes. 44.5% after 60 minutes in CMF. The percentage of intracellular organisms were 82% after 30 minutes, 90% after 60 minutes in normal rabbit serum, and 73% after 30 minutes, 9395 after 60 minutes in CMF. 3. In the bacterial mixture-macrophage suspension; The percentages of macrophages with intracellular organisms were 30% after 30 minutes, 35% after 60 minutes in normal rabbit serum, and 53.59 after 30 minutes 57.5% after 60 minutes in CMF. The percentages of intracellular organisms were 87.5% after 30 minutes, 93% after 60 minutes in normal rabbit serum, and 77% after 30 minutes, 87% after 60 minutes in CMF.

MeSH Terms

In Vitro Techniques*
Macrophages*
Suspensions
Suspensions

Figure

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