J Dent Hyg Sci.  2019 Dec;19(4):288-293. 10.17135/jdhs.2019.19.4.288.

Effects of Bamboo Salt with Sodium Fluoride on the Prevention of Dental Caries

Affiliations
  • 1Department of Dental Hygiene, Howon University, Gunsan 54058, Korea. qorrha12@daum.net
  • 2Department of Dental Hygiene, Wonkwang Health Science University, Iksan 54538, Korea.

Abstract

BACKGROUND
Dental caries is one of several prevalent oral diseases caused by dental plaque biofilms. This study evaluated the anti-cariogenic effects of a bamboo salt (BS) and sodium fluoride (NaF) mixture on oral bacteria.
METHODS
The effects of several mixtures of NaF and BS on acid production, growth, and adhesion to glass beads of Streptococcus mutans, and their anti-cariogenic properties were investigated. The growth of S. mutans was measured according to optical density at 3, 6, 9, 12, 15, 18, and 24 hours after treatment using spectrophotometry at a wavelength of 600 nm, while pH was measured using a pH meter. Adhesion of S. mutans was measured according to the weight of glass beads from each group before and after incubation. Gene expression was measured using real-time polymerase chain reaction. Acid production and growth patterns of S. mutans were compared using repeated measures analysis of variance, followed by Scheffe's post-hoc test. The Kruskal-Wallis test was used to compare adhesion, followed by the Mann-Whitney test. Gene expression in the experimental and control samples was compared using the Student's t-test.
RESULTS
Growth, acid production, and adhesion of S. mutans were inhibited in all experimental groups. Expression of gft and fructosyltransferase in S. mutans was inhibited in all groups. A mixture of NaF and BS significantly reduced growth, acid production, adhesion, and gene expression of S. mutans compared with the other groups.
CONCLUSION
Results of the present study demonstrated that a mixture of NaF and BS was useful as a mouth rinse in preventing dental caries.

Keyword

Bamboo salt; Dental caries; Sodium fluoride; Streptococcus mutan
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