Allergy Asthma Immunol Res.  2020 Mar;12(2):364-370. 10.4168/aair.2020.12.2.364.

Primary Nasal Epithelial Cells From Allergic and Non-allergic Individuals Show Comparable Barrier Function

Affiliations
  • 1Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria. verena.niederberger@meduniwien.ac.at
  • 2Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
  • 3NRC Institute of Immunology FMBA of Russia, Moscow, Russia.
  • 4Laboratory for Immunopathology, Department of Clinical Immunology and Allergy, Sechenov First Moscow State Medical University, Moscow, Russia.

Abstract

Previous reports suggested that ex vivo cultured primary nasal epithelial cells from allergic patients differ from those from non-allergic individuals by genuinely reduced barrier function. By contrast, we found that primary nasal epithelial cells from allergic and non-allergic individuals showed comparable barrier function and secretion of cytokines.

Keyword

Respiratory epithelial barrier function; allergic rhinitis; allergen; immunoglobulin E; allergen-microarray; transepithelial resistance

MeSH Terms

Cytokines
Epithelial Cells*
Humans
Immunoglobulin E
Rhinitis, Allergic
Cytokines
Immunoglobulin E

Figure

  • Fig. 1 Transepithelial resistance and impedance of cultured primary human nasal epithelial cells from non-allergic and allergic subjects. (A) Epithelial barrier function was measured by analyzing TER after 21 days of ALI cultured cells. (B) Impedance-based values were obtained after 7 days in submerged cultures. Samples were extracted from inferior turbinates from allergic (right panel; squares) and non-allergic (left panel; dots) patients. Smoking subjects are labeled in red. Statistical significance analyses were performed with the unpaired t-test. Standard deviation values are visualized as error bars. TER, trans-epithelial electrical resistance; ALI, air-liquid interface.

  • Fig. 2 Cytokine levels of supernatants from cultured primary human nasal epithelial cells obtained from non-allergic and allergic subjects. On the left are the results from 13 non-allergic individuals (dots). To the right are the results from 9 allergic patients (squares). Proinflammatory cytokines that were measured after 7, 14, 21 and 28 days of culture include GM-CSF (A), IL-33 (B), RANTES (C), IL-1α (D) and TSLP (E). A different scale is used in A as a higher level of GM-CSF is expressed compared with the other cytokines. GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; RANTES, regulated on activation, normal T cell expressed and secreted; TSLP: thymic stromal lymphopoietin.


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