J Korean Assoc Oral Maxillofac Surg.  2018 Dec;44(6):259-268. 10.5125/jkaoms.2018.44.6.259.

Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts

• Jeong SY
• Hong JU
• Song JM
• Kim IR
• Park BS
• Kim CH
• Shin SH

• KMID: 2467172
• DOI: http://doi.org/10.5125/jkaoms.2018.44.6.259

Abstract

OBJECTIVES
The purpose of this study was to evaluate the synergic effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and low-level laser therapy (LLLT) on bisphosphonate-treated osteoblasts.
MATERIALS AND METHODS
Human fetal osteoblast cells (hFOB 1.19) were cultured with 100 µM alendronate. Low-level Ga-Al-As laser alone or with 100 ng/mL rhBMP-2 was then applied. Cell viability was measured with MTT assay. The expression levels of receptor activator of nuclear factor kappa-B ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG) were analyzed for osteoblastic activity inducing osteoclastic activity. Collagen type and transforming growth factor beta-1 were also evaluated for bone matrix formation.
RESULTS
The results showed that rhBMP-2 and LLLT had a synergic effect on alendronate-treated osteoblasts for enhancing osteoblastic activity and bone matrix formation. Between rhBMP-2 and LLLT, rhBMP-2 exhibited a greater effect, but did not show a significant difference.
CONCLUSION
rhBMP-2 and LLLT have synergic effects on bisphosphonate-treated osteoblasts through enhancement of osteoblastic activity and bone formation activity.

Keyword

Alendronate; Osteoblasts; Bone morphogenetic protein 2; Low-level laser therapy

MeSH Terms

Alendronate
Bone Matrix
Bone Morphogenetic Protein 2
Cell Survival
Collagen
Humans*
Low-Level Light Therapy
Macrophage Colony-Stimulating Factor
Osteoblasts*
Osteoclasts
Osteogenesis
Osteoprotegerin
Transforming Growth Factors
Alendronate
Bone Morphogenetic Protein 2
Collagen
Macrophage Colony-Stimulating Factor
Osteoprotegerin
Transforming Growth Factors