Korean J Physiol Pharmacol.  2020 Jan;24(1):101-110. 10.4196/kjpp.2020.24.1.101.

Analysis of interaction between intracellular spermine and transient receptor potential canonical 4 channel: multiple candidate sites of negatively charged amino acids for the inward rectification of transient receptor potential canonical 4

Affiliations
  • 1Department of Physiology, College of Medicine, Seoul National University, Seoul 03080, Korea. insuk@snu.ac.kr
  • 2Office of Medical Education, College of Medicine, Seoul National University, Seoul 03080, Korea.
  • 3Department of Surgery, College of Medicine, Seoul National University, Seoul 03080, Korea.
  • 4Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

Abstract

Transient receptor potential canonical 4 (TRPC4) channel is a nonselective calcium-permeable cation channels. In intestinal smooth muscle cells, TRPC4 currents contribute more than 80% to muscarinic cationic current (mIcat). With its inward-rectifying current-voltage relationship and high calcium permeability, TRPC4 channels permit calcium influx once the channel is opened by muscarinic receptor stimulation. Polyamines are known to inhibit nonselective cation channels that mediate the generation of mIcat. Moreover, it is reported that TRPC4 channels are blocked by the intracellular spermine through electrostatic interaction with glutamate residues (E728, E729). Here, we investigated the correlation between the magnitude of channel inactivation by spermine and the magnitude of channel conductance. We also found additional spermine binding sites in TRPC4. We evaluated channel activity with electrophysiological recordings and revalidated structural significance based on Cryo-EM structure, which was resolved recently. We found that there is no correlation between magnitude of inhibitory action of spermine and magnitude of maximum current of the channel. In intracellular region, TRPC4 attracts spermine at channel periphery by reducing access resistance, and acidic residues contribute to blocking action of intracellular spermine; channel periphery, E649; cytosolic space, D629, D649, and E687.

Keyword

Polyamines; Spermine; Transient receptor potential channels
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