Infect Chemother.  2019 Dec;51(4):365-375. 10.3947/ic.2019.51.4.365.

Characteristics of Faecal Microbiota in Korean Patients with Clostridioides difficile-associated Diarrhea

Affiliations
  • 1Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. seran@yuhs.ac
  • 2AIDS Research Institute, Yonsei University College of Medicine, Seoul, Korea.
  • 3Department of Internal Medicine, Kangwon National University School of Medicine, Chuncheon, Korea.
  • 4Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.
  • 5Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
The intestinal microbiota plays an important role in the pathogenesis of Clostridioides difficile-associated diarrhea, and regional and racial characteristics influence the microbiome composition and diversity. We investigated the intestinal microbiome characteristics of patients with C. difficile colitis (CD+) compared to those of patients with colitis not due to C. difficile (CD−), patients with vancomycin-resistant enterococci (VRE) colonization, and healthy controls, in Korea.
MATERIALS AND METHODS
We collected stool samples from 24, 18, 11 and 13 subjects within CD+, CD−, VRE and healthy control groups, respectively. The microbial communities were evaluated by 454-pyrosequencing of bacterial 16s rRNA.
RESULTS
The species richness and microbial diversity were significantly lower in the CD+ group compared to those in healthy controls, but not compared to those in CD− and VRE groups. Phylum-level analysis showed that the proportion of Actinobacteria in the CD+ group was significantly lower than in the healthy control, but was unchanged compared to that in CD− and VRE groups. At the genus level, compared to the healthy group, the CD+ group showed significantly lower proportions of Blautia, Bifidobacterium, Faecalibacterium et al. Compared to the VRE group, the CD+ group showed a significantly higher proportion of Anaerostipes.
CONCLUSIONS
We could identify the intestinal microbiome characteristics of Koreans with C. difficile colitis. It might help to develop microbiome based diagnostic and treatment modalities.

Keyword

Faecal microbiota; Intestinal microbiota; Clostridioides difficile infection; Next generation sequencing

MeSH Terms

Actinobacteria
Bifidobacterium
Colitis
Colon
Diarrhea*
Gastrointestinal Microbiome
Humans
Korea
Microbiota*
Vancomycin-Resistant Enterococci
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