Yonsei Med J.  2020 Jan;61(1):56-63. 10.3349/ymj.2020.61.1.56.

Effect of Dialysis on Aryl Hydrocarbon Receptor Transactivating Activity in Patients with Chronic Kidney Disease

  • 1Department of Internal Medicine, Nowon Eulji University Hospital, Seoul, Korea. leesy1146@eulji.ac.kr
  • 2Department of Internal Medicine, Kangwon National University School of Medicine, Chuncheon, Korea.
  • 3Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea. ykpak@khu.ac.kr


Elevated aryl hydrocarbon receptor (AhR) transactivating (AHRT) activity and uremia in chronic kidney disease (CKD) may interact with each other, further complicating the disease course. In this study, we prospectively estimated serum AHRT activity using a highly sensitive cell-based AhR-dependent luciferase activity assay in CKD patients and compared differences therein according to treatment modality.
Patients undergoing peritoneal dialysis (PD) (n=22) and hemodialysis (HD) (n=38) and patients with pre-dialysis CKD stage IV or V (n=28) were included. AHRT activity and intracellular adenosine triphosphate (ATP) levels were measured. We performed a correlation analysis for AHRT activity, ATP levels, and various clinical parameters.
AHRT activity and intracellular ATP levels were inversely correlated and differed according to treatment modalities. AHRT activity was higher in non-dialysis CKD patients than in patients undergoing dialysis and was higher in patients undergoing HD, compared to PD. AHRT activity decreased after HD treatment in HD patients. ATP levels were higher in healthy controls than in patients with pre-dialysis CKD and PD and were further decreased in patients with HD. We noted significant correlations between multiple clinical parameters associated with cardiovascular risk factors and AHRT activity.
AHRT activity was elevated in CKD patients, while dialysis treatment reduced AHRT activity. Further studies are warranted to specify AHRT activity and to evaluate the precise roles thereof in patients with CKD.


Aryl hydrocarbon receptor transactivating activity; chronic kidney disease; dialysis

MeSH Terms

Adenosine Triphosphate
Peritoneal Dialysis
Prospective Studies
Receptors, Aryl Hydrocarbon*
Renal Dialysis
Renal Insufficiency, Chronic*
Risk Factors
Adenosine Triphosphate
Receptors, Aryl Hydrocarbon


  • Fig. 1 Comparison of AHRT activity and intracellular ATP levels among study groups. Significance at p<0.05 by ANOVA and Tukey's test . *p<0.001, †p<0.05. AHRT, aryl hydrocarbon receptor transactivating; ATP, adenosine triphosphate; CKD, chronic kidney disease; PD, peritoneal dialysis; HD, hemodialysis.

  • Fig. 2 Comparison of AHRT activity and ATP levels according to DM status. *p<0.001, †p<0.05. AHRT, aryl hydrocarbon receptor transactivating; ATP, adenosine triphosphate; DM, diabetes mellitus.

  • Fig. 3 Changes in AHRT activity and intracellular ATP levels after HD treatment in HD patients. Paired-t test was used. AHRT, aryl hydrocarbon receptor transactivating; ATP, adenosine triphosphate; HD, hemodialysis.

  • Fig. 4 Significant correlations among AHRT activity and various clinical parameters. Pearson's coefficient r and p values are presented. AHRT, aryl hydrocarbon receptor transactivating; ATP, adenosine triphosphate; BMI, body mass index; LDL, low-density-lipoprotein; SBP, systolic blood pressure; LVEF, left ventricular ejection fraction; FI, fold induction.


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