Korean J Parasitol.  2019 Oct;57(5):481-487. 10.3347/kjp.2019.57.5.481.

Mitochondrial DNA Sequence Variability of Spirometra Species in Asian Countries

Affiliations
  • 1Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju 28644, Korea. kseom@chungbuk.ac.kr

Abstract

Mitochondrial DNA sequence variability of Spirometra erinaceieuropaei in GenBank was observed by reinvestigation of mitochondrial cox1 and cytb sequences. The DNA sequences were analyzed in this study, comprising complete DNA sequences of cox1 (n=239) and cytb (n=213) genes. The 10 complete mitochondrial DNA sequences of Spirometra species were compared with those of Korea, China and Japan. The sequences were analyzed for nucleotide composition, conserved sites, variable sites, singleton sites and parsimony-informative sites. Phylogenetic analyses was done using neighbor joining, maximum parsimony, Bayesian inference and maximum-likelihood on cox1 and cytb sequences of Spirometra species. These polymorphic sites identified 148 (cox1) and 83 (cytb) haplotypes within 239 and 213 isolates from 3 Asian countries. Phylogenetic tree topologies were presented high-level confidence values for the 2 major branches of 2 Spirometra species containing S. erinaceieuropaei and S. decipiens, and S. decipiens sub-clades including all sequences registered as S. erinaceieuropaei in cox1 and cytb genes. These results indicated that mitochondrial haplotypes of S. erinaceieuropaei and S. decipiens were found in the 3 Asian countries.

Keyword

Spirometra erinaceieuropaei; Spirometra decipiens; mitochondria; DNA sequence variability

MeSH Terms

Asian Continental Ancestry Group*
Base Sequence
China
Databases, Nucleic Acid
DNA, Mitochondrial*
Haplotypes
Humans
Japan
Korea
Mitochondria
Spirometra*
Trees
DNA, Mitochondrial
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