Asia Pac Allergy.
2019 Oct;9(4):e32. 10.5415/apallergy.2019.9.e32.
Respiratory syncytial virus increases eosinophil extracellular traps in a murine model of asthma
- Affiliations
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- 1Department of Biochemistry, Laboratory of Neuroprotection and Neurometabolic Disease, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
- 2Laboratory of Pediatric Respirology - Infant Center, PontifÃcia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil. alineacunha@hotmail.com
- 3Laboratory of Cellular and Molecular Immunology, PontifÃcia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil.
- 4Laboratory of Neuroscience - Brain Institute, PontifÃcia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil.
- 5Hospital Moinhos de Vento, Porto Alegre, Rio Grande do Sul, Brazil.
- KMID: 2461104
- DOI: http://doi.org/10.5415/apallergy.2019.9.e32
Abstract
- BACKGROUND
Respiratory viral infections are the leading cause of asthma exacerbations. Eosinophil activation results in the formation of eosinophil extracellular traps (EETs), which release web-like structures of DNA and proteins that bind, disarm and extracellularly kill pathogens.
OBJECTIVE
We investigated whether the respiratory syncytial virus (RSV) in vitro could induce EETs in bronchoalveolar lavage fluid eosinophils in a murine model of asthma.
METHODS
BALB/cJ mice (6-8 weeks old) were sensitized with 2 subcutaneous injections of ovalbumin (20 μg) on days 0 and 7, followed by three intranasal challenges with ovalbumin (100 μg) on days 14, 15, and 16 of the protocol. The control group received Dulbecco's phosphate-buffered saline. Bronchoalveolar lavage fluid eosinophils of ovalbumin group or control group were stimulated with RSV (103 PFU/mL) in vitro for 3 hours. After that, culture supernatant was collected to perform the analyses proposed in this study.
RESULTS
We verified an increase in extracellular DNA concentration in bronchoalveolar lavage fluid eosinophils from ovalbumin group stimulated with RSV (10³ PFU/mL) in vitro, which was confirmed by confocal microscopy. We demonstrated that most cells are negative for annexin V and propidium iodide in all groups evaluated. Also, RSV in vitro decreased interferon-É£ in culture supernatant when compared to the ovalbumin group.
CONCLUSION
In this study, we demonstrated for the first time that RSV in vitro induces EETs formation in eosinophils from asthmatic mice.
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