Nat Prod Sci.  2019 Sep;25(3):255-260. 10.20307/nps.2019.25.3.255.

Anti-oxidative Effect of Piperine from Piper nigrum L. in Caenorhabditis elegans

Affiliations
  • 1College of Pharmacy, Woosuk University, Jeonju 55338, Republic of Korea. dkkim@woosuk.ac.kr

Abstract

Piper nigrum L. (Piperaceae), which is a well-known food seasoning, has been used as a traditional medicine for the treatment of vomiting, abdominal pain, diarrhea and anorexia in Korea, China and Japan. Methanol extract from the fruit of P. nigrum was successively partitioned as n-hexane, methylene chloride, ethyl acetate, n-butanol and Hâ‚‚O soluble fractions. Among those fractions the ethyl acetate soluble fraction showed the most potent DPPH radical scavenging activity, and piperine was isolated from the ethyl acetate fraction. To know the antioxidant activity of piperine, we tested the activities of superoxide dismutase (SOD) and catalase together with oxidative stress tolerance and intracellular ROS level in Caenorhabditis elegans. To investigate whether piperine-mediated increased stress tolerance was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain including CF1553. Consequently, piperine enhanced SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, piperine-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

Keyword

Piperine; Piper nigrum L.; Caenorhabditis elegans; Antioxidant activity

MeSH Terms

1-Butanol
Abdominal Pain
Anorexia
Caenorhabditis elegans*
Caenorhabditis*
Catalase
China
Diarrhea
Fruit
Japan
Korea
Medicine, Traditional
Methanol
Methylene Chloride
Oxidative Stress
Piper nigrum*
Piper*
Seasons
Superoxide Dismutase
Vomiting
1-Butanol
Catalase
Methanol
Methylene Chloride
Superoxide Dismutase
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