Lab Anim Res.  2018 Dec;34(4):311-316. 10.5625/lar.2018.34.4.311.

Genetic and morphometric characteristics of Korean wild mice (KWM/Hym) captured at Chuncheon, South Korea

Affiliations
  • 1Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Korea. jgsuh@hallym.ac.kr
  • 2Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Korea.

Abstract

Laboratory inbred mice are used widely and commonly in biomedical research, but inbred mice do not have a big enough gene pool for the research. In this study, genetic and morphometric analyses were performed to obtain data on the characteristics of a newly developing inbred strain (KWM/Hym) captured from Chuncheon, Korea. All of five Korean wild male mice have the zinc-finger Y (ZfY) gene. Also, all of 19 Korean wild mice used in this analysis have the AKV-type murine leukemia virus gene, indicating that Korean wild mice might be Mus musculus musculus. To identify the genetic polymorphism in KWM/Hym, SNP analysis was performed. In a comparison with 28 SNP markers, there was a considerable difference between KWM/Hym and several inbred strains. The homogeneity between KWM/Hym and the inbred strains was as follows: C57BL/6J (39.3%), BALB/c AJic (42.9%), and DBA/2J (50%). KWM/Hym is most similar to the PWK/PhJ inbred strain (96.4%) derived from wild mice (Czech Republic). To identify the morphometric characteristics of KWM/Hym, the external morphology was measured. The tail ratio of male and female was 79.60±3.09 and 73.55±6.14%, respectively. KWM/Hym has short and agouti-colored hairs and its belly is white with golden hair. Taking these results together, KWM/Hym, a newly developing inbred mouse originated from wild mouse, might be use as new genetic resources to overcome the limitations of the current laboratory mice.

Keyword

Korean wild mouse; Mus musculus; subspecies; polymorphism; genetic resources

MeSH Terms

Animals
Female
Gangwon-do*
Gene Pool
Hair
Humans
Korea*
Leukemia Virus, Murine
Male
Mice*
Polymorphism, Genetic
Tail

Figure

  • Figure 1 Distribution of ZfY and AKV-type MuLV genes in Korean wild mice. Bands of 202 and 184 bp and 530 bp indicate distribution of Zfy and AKV-type MuLV genes, respectively. Mark: 100 bp DNA ladder.

  • Figure 2 Photograph and coat color of Korean wild mice. (A) Photograph of Korean wild mouse. Korean wild mouse has agouti-colored back and white belly with golden hair. (B) The four main hair types. Monotrich (a), awl (b), auchen (c), and zigzag (d).


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