J Gastric Cancer.  2019 Sep;19(3):315-328. 10.5230/jgc.2019.19.e28.

Combined Detection of Serum MiR-221-3p and MiR-122-5p Expression in Diagnosis and Prognosis of Gastric Cancer

Affiliations
  • 1Department of Gastroenterology, Taizhou First People's Hospital, Taizhou 318020, China.
  • 2Department of General Surgery, Taizhou First People's Hospital, Taizhou 318020, China. liaonansheng2019@163.com

Abstract

PURPOSE
To investigate the clinical value of serum miR-221-3p and miR-122-5p expression levels in the diagnosis and prognosis of gastric cancer.
MATERIALS AND METHODS
Serum samples from 141 gastric cancer cases (gastric cancer group), 110 gastric polyps (gastric polyp group), and 75 healthy people (healthy control) were used to detect miR-221-3p and miR-122-5p expression using real-time reverse transcription polymerase chain reaction.
RESULTS
Serum miR-221-3p expression was significantly higher in the gastric cancer group than in the gastric polyp group, and it was significantly lower than that before operation. The miR-221-3p expression was significantly higher in the death group than in the survival group. The proliferation and migration ability significantly increased and the apoptosis rate significantly decreased by miR-221-3p transfection in gastric cancer cells. In contrast, the function of miR-122-5p in gastric cancer cells was opposite of miR-221-3p. Serum miR-221-3p expression was negatively correlated with that of miR-122-5p in gastric cancer. Serum miR-221-3p and miR-122-5p expressions were significantly correlated with the degree of differentiation, tumor, node, metastasis stage, lymph node metastasis, and invasion depth. miR-221-3p and miR-122-5p expression levels were independent prognostic factors for postoperative gastric cancer. In the diagnosis and predicting prognosis of gastric cancer, receiver operating characteristic analysis revealed that the area under curve of combined detection of serum miR-221-3p and miR-122-5p expression had a greater diagnostic effect than either single maker.
CONCLUSIONS
The miR-221-3p and miR-122-5p are involved in the development of gastric cancer, and they have important clinical values in gastric cancer diagnosis and prognosis.

Keyword

MicroRNAs; Gastric cancer; Diagnosis; Prognosis; Real-time polymerase chain reaction

MeSH Terms

Apoptosis
Area Under Curve
Diagnosis*
Lymph Nodes
MicroRNAs
Neoplasm Metastasis
Polymerase Chain Reaction
Polyps
Prognosis*
Real-Time Polymerase Chain Reaction
Reverse Transcription
ROC Curve
Stomach Neoplasms*
Transfection
MicroRNAs
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