J Korean Acad Oral Health.  2019 Jun;43(2):72-77. 10.11149/jkaoh.2019.43.2.72.

Effect of apoptosis on G361 cells by Cimicifuga rhizoma extract

  • 1Feagle Co., Ltd., Yangsan, Korea.
  • 2Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan, Korea.
  • 3Department of Korean Internal Medicine, Pusan National University, Yangsan, Korea. jwhong@pusan.ac.kr
  • 4Department of Dental Hygiene, Kyungnam College of Information & Technology, Busan, Korea. sanglye5@naver.com


To investigate whether the cytotoxic effect of Cimicifuga rhizoma extract is associated with cell death in the human keratinocyte (HaCaT) and human melanoma cell lines (G361).
Apoptosis induced by Cimicifuga rhizoma extract was confirmed by water-soluble tetrazolium salts-1 (WST-1) assay, immunocytochemistry, and western blot. Additionally, the release of cytochrome c and apoptosis-inducing factor (AIF) was visualized by confocal laser scanning microscopy.
The results showed that Cimicifuga rhizoma extract significantly reduced the viability of G361 cells with half-maximal inhibitory concentration (IC 50) of 200 µg/ml, and the apoptotic process was found to occur via the activation of caspase-3 and caspase-9 pathways. Besides, the release of cytochrome c and AIF was also detected.
This study suggests that Cimicifuga rhizoma extract causes apoptosis of human melanoma cells through the intrinsic apoptotic pathway.


Apoptosis; Cimicifuga rhizoma; Melanoma
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