Lab Anim Res.  2019 Jun;35(1):13-22. 10.1186/s42826-019-0003-3.

Efficacy and local irritation evaluation of Eriobotrya japonica leaf ethanol extract

Affiliations
  • 1Health Care Institute, Korea Testing and Research Institute, Hwasun, Jeonnam 58141, Republic of Korea.
  • 2College of Veterinary Medicine (BK21 Plus Project Team), Chonnam National University, Gwangju 61186, Republic of Korea. toxkim@jnu.ac.kr

Abstract

BACKGROUND
Although Eriobotrya japonica leaves have been studied as a raw material for various cosmetic products, little is known about the anti-oxidant, anti-inflammatory, and anti-melanogenic activities of Eriobotrya japonica leaf ethanol extract (EJEE).
METHODS
This study was conducted to evaluate the anti-oxidant, anti-inflammatory, and anti-melanogenic activities of EJEE using different in vitro models. In addition, we investigated the potential irritation of EJEE to skin and eye using animal alternative tests.
RESULTS
The total content of polyphenols, one of the active constituents of EJEE, was analyzed by high-performance liquid chromatography and found to contain 88.68 mg tannic acid equivalent/g. EJEE showed a concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, 2,2"²-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging activity, and a superoxide dismutase-like activity. The anti-inflammatory effect of 0.5% (w/v) EJEE was demonstrated by a reduction in lipopolysaccharide-induced nitric oxide and tumor necrosis factor-alpha levels in RAW 264.7 cells. EJEE also significantly inhibited melanogenesis in melanocyte stimulating hormone-induced B16F1 cells. EJEE did not show any irritation in skin and eye in animal alternative test.
CONCLUSIONS
These results indicate that the EJEE possesses anti-oxidant, anti-inflammatory, and anti-melanogenic activities, while it did not induce toxicity or irritation in neither skin nor eye. Therefore, EJEE can be used as a cosmetic ingredient for skin improvement.

Keyword

Eriobotrya japonica leaf ethanol extract; Anti-oxidant activity; Anti-inflammatory activity; Anti-melanogenic activity; Local irritation; Animal alternative test

MeSH Terms

Animals
Chromatography, Liquid
Eriobotrya*
Ethanol*
In Vitro Techniques
Melanocytes
Nitric Oxide
Polyphenols
RAW 264.7 Cells
Skin
Superoxides
Tannins
Tumor Necrosis Factor-alpha
Ethanol
Nitric Oxide
Polyphenols
Superoxides
Tannins
Tumor Necrosis Factor-alpha
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