J Nutr Health.  2019 Aug;52(4):408-411. 10.4163/jnh.2019.52.4.408.

Effect of Yeonsan Ogye bioactive peptides on anti-oxidant indexes in rats' liver

Affiliations
  • 1Department of Food and Nutrition, Anyang University, Anyang, Gyeonggi 14028, Korea.
  • 2Institute of Health and Nutrition, Anyang, Gyeonggi 14028, Korea.
  • 3Department of Food and Nutrition, Daejeon University, Daejeon 34520, Korea. kimkinam@dju.kr

Abstract

PURPOSE
This study investigated the effect of bioactive Yeonsan Ogye peptides (YOPs) intake on changes in the hepatic anti-oxidant indexes in male rats.
METHODS
Sprague-Dawley male rats were divided into 3 groups and given a casein-based AIN-93G diet and distilled water ad libitum without any added YOPs (control), distilled water with 250 mg of YOPs (Y250), or 500 mg of YOPs (Y500) per kg of body weight for 4 weeks. YOP dose was decided as referred to in the referenced study where toxicity did not occur. The hepatic anti-oxidant indexes were determined using a commercial kit. Statistical analysis was performed using SPSS version 23.0 and are expressed as mean ± standard error of mean. Differences among the groups were evaluated by one-way analysis of variance followed by post hoc Duncan's multiple comparisons test.
RESULTS
There were no differences in the body weights, weight gain, food intake, food efficiency ratio, or organ weight, including liver, kidney, spleen, thymus, and epididymal fat, among all of the groups. The hepatic nitric oxide (NO) level in the Y500 group was lower than that in the control and Y250 groups, and the hepatic malondialdehyde (MDA) level was lower in the Y500 group than in the Y250 group. The differences in hepatic superoxide dismutase (SOD) and catalase (CAT) activities were not statistically significant between the groups. From these results we speculated that YOPs may have anti-oxidative abilities to regulate NO and MDA production without affecting SOD and CAT activities.
CONCLUSION
YOPs are presumed to act as anti-oxidants in the animal or human body.

Keyword

anti-oxidants; bioactive peptides; liver; Sprague-Dawley rats; Yeonsan Ogye

MeSH Terms

Animals
Body Weight
Catalase
Cats
Diet
Eating
Human Body
Humans
Kidney
Liver*
Male
Malondialdehyde
Nitric Oxide
Organ Size
Peptides*
Rats
Rats, Sprague-Dawley
Spleen
Superoxide Dismutase
Thymus Gland
Water
Weight Gain
Catalase
Malondialdehyde
Nitric Oxide
Peptides
Superoxide Dismutase
Water

Figure

  • Fig. 1 Hepatic level of (A) NO and (B) MDA and hepatic activity of (C) SOD and (D) CAT of rats fed with experimental diets for 4 weeks. C, control group; Y250, fed with distilled water + YOPs containing 250 mg/kg of body weight; Y500, fed with distilled water + YOPs containing 500 mg/kg of body weight. Values are expressed as means (n = 9 per group). Significant differences among 3 groups were analyzed by one-way ANOVA followed by post hoc Duncan's multiple comparisons test.


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