Nat Prod Sci.  2019 Jun;25(2):157-164. 10.20307/nps.2019.25.2.157.

Evaluation of Acute and Sub-acute Oral Toxicity Effect of Aquilaria malaccensis Leaves Aqueous Extract in Male ICR Mice

• Musa NH
• Zain HH
• Ibrahim H
• Jamil NN

• KMID: 2452786
• DOI: http://doi.org/10.20307/nps.2019.25.2.157

Abstract

The study was conducted to investigate the acute and sub-acute toxicity effect of Aquilaria malaccensis leaves aqueous extract (AEAM) towards male ICR mice in terms of body weight, relative organ weight, mortality rate and sperm parameters. In acute toxicity study, a single dose at of 2000 mg/kg was performed. In sub-acute toxicity study, the mice were received normal saline (control group), 50, 100, 150, 200, 500, or 1000 mg/kg of AEAM orally for 21 days of treatment. In sub-acute toxicity study, the number of abnormal sperm were significantly decreased in AEAM 100, 150, 200, 500, and 1000 when compared to the control group. While, the motility of sperm were found to be significantly increased in AEAM 100, 150, 200, and 1000 as compared to the control group. No mortality was recorded in the control group and treated groups in both toxicity studies except for one mouse from AEAM 1000 group. However, the mild sedative effect in terms of the tendency to sleep was clearly noticeable in both toxicity studies. Results indicated that the AEAM can be one of the useful alternative medicine to enhance fertility rate by increasing healthy sperm production.

Keyword

Aquilaria malaccensis; Aqueous extract; Acute toxicity; Sub-acute toxicity

MeSH Terms

Animals
Birth Rate
Body Weight
Complementary Therapies
Humans
Hypnotics and Sedatives
Male*
Mice
Mice, Inbred ICR*
Mortality
Organ Size
Spermatozoa
Thymelaeaceae*
Hypnotics and Sedatives

Figure

• 

  Fig. 1. Sperm morphology of ICR male mice, as indicated by eosin staining and observed using inverted microscope (40×magnification),(A) Normal sperm, (B) No hook, (C) Pin head, (D) Bent head, (E) Coiled flagellum, (F) Hairpin loop, (G) Bent flagellum.

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