J Bacteriol Virol.  2018 Dec;48(4):147-155. 10.4167/jbv.2018.48.4.147.

Genome Sequencing of Human Cytomegalovirus Strain LCW and Identification of Asia-specific Cluster

• Kang JH
• Yoon JH
• Hwang HR
• Lee CH

• KMID: 2452067
• DOI: http://doi.org/10.4167/jbv.2018.48.4.147

Abstract

Human cytomegalovirus (HCMV) is a ubiquitous human pathogen and contains double stranded DNA genome with approximately 230 kbp. Molecular genomic studies of HCMV have been attempted in order to understand the pathogenesis and evolution of HCMV. However, studies on HCMV strains of Asian origin are limited. In this study, it was attempted to understand the genomics of HCMV isolated from Korea. Clinical strain LCW isolated from Korean patient was passaged in vitro cell culture, and subjected to next-generation sequencing. Complete genome sequence was obtained and compared with other HCMV strains. The LCW genome was found to contain 170 open reading frames (ORFs) and two ORF (RL5A and RL13) of the strain LCW were found to be truncated due to early stop codon. Phylogenetic analysis suggested that the strain LCW was closely related with Asian strains such as HCMV strains JHC and HAN. Common nucleotide sequences among the 3 Asian strains distinguishable from other strains were detected at 197 sites including 104 sites in ORFs.

Keyword

Human cytomegalovirus; Genome sequence; Asian cluster

MeSH Terms

Animals
Asian Continental Ancestry Group
Base Sequence
Cell Culture Techniques
Codon, Terminator
Cytomegalovirus*
DNA
Ecthyma, Contagious
Genome*
Genomics
Humans*
In Vitro Techniques
Korea
Open Reading Frames
Codon, Terminator
DNA

Figure

• 

  Figure 1 Genome map of the HCMV strain LCW. Nucleotide positions corresponding to the HCMV genome structure and the ORFs contained in each of the genome structure is indicated.

• 

  Figure 2 Sequence alignment of HCMV ORF RL5A and RL13. Early stops due to mutations in the strain LCW are boxed. (A) ORF RL5A due to T->A substitution at the third position of the codon, (B) RL13 due to C->A substitution at the first position of the codon.

• 

  Figure 3 Phylogenetic tree of 42 HCMV strains. Genomic DNA sequences of HCMV strains were retrieved from NCBI GenBank database and aligned with ClustalW program together with the genome sequence of the Korean strain LCW. Phylogenetic tree was constructed using neighbor-joining model in Phylip package. Cluster of HCMV strains of Asian origin is indicated as a circle.

• 

  Figure 4 Distribution of the mutations found specifically in Asian strains of HCMV. Numbers of synonymous (□) and nonsynonymous (■) mutations are shown.

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