Ann Clin Microbiol.  2019 Jun;22(2):42-49. 10.5145/ACM.2019.22.2.42.

Antimicrobial Susceptibility and Genetic Analysis of Campylobacter jejuni Isolated from Diarrhea Patients in Busan

• Park SH
• Kim BJ
• Kwon YH
• Hwang IY
• Sung GH
• Park EH
• Jin SH

• KMID: 2450002
• DOI: http://doi.org/10.5145/ACM.2019.22.2.42

Abstract

BACKGROUND
Campylobacter jejuni is an important food-borne pathogen that causes human gastroenteritis. This study was conducted to investigate the incidence of isolation, antimicrobial susceptibility pattern, and C. jejuni genotype from diarrhea patients in Busan, Korea.
METHODS
A total of 97 C. jejuni were isolated from diarrhea patients during five food-borne outbreaks from 2014 to September 2017. Antimicrobial susceptibility tests were carried out by the broth microdilution method for ciprofloxacin (CIP), nalidixic acid (NAL), tetracycline (TET), chloramphenicol, azithromycin (AZI), erythromycin (ERY), streptomycin (STR), gentamicin, and telithromycin. To investigate C. jejuni genotypes, pulsed-field gel electrophoresis (PFGE) profile analysis was performed.
RESULTS
The isolation rate of C. jejuni was 2.0% for the last 4 years and increased annually. Antimicrobial resistance rates of C. jejuni were shown to be in the order of NAL (90.9%), CIP (89.4%), TET (13.6%), AZI (3.0%), ERY (3.0%), and STR (1.5%). The proportion of multidrug-resistance was 18.2%, and they commonly contained quinolones (CIP-NAL). Analysis of PFGE patterns of SmaI-restricted DNA of C. jejuni isolates showed 17 clusters; cluster 11 was the major genotype pattern.
CONCLUSION
This study will provide useful data for the proper use of antimicrobials and the management of resistant C. jejuni. Also it will help to provide data for the epidemiological investigation of foodborne diseases caused by C. jejuni, which is expected to increase in the future.

Keyword

Antimicrobial susceptibility; Campylobacter jejuni; Pulsed-field gel electrophoresis

MeSH Terms

Azithromycin
Busan*
Campylobacter jejuni*
Campylobacter*
Chloramphenicol
Ciprofloxacin
Diarrhea*
Disease Outbreaks
DNA
Electrophoresis, Gel, Pulsed-Field
Erythromycin
Foodborne Diseases
Gastroenteritis
Genotype
Gentamicins
Humans
Incidence
Korea
Methods
Nalidixic Acid
Quinolones
Streptomycin
Tetracycline
Azithromycin
Chloramphenicol
Ciprofloxacin
DNA
Erythromycin
Gentamicins
Nalidixic Acid
Quinolones
Streptomycin
Tetracycline

Figure

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  Fig. 1 Prevalence of C. jejuni isolates from diarrhea patients by month.

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  Fig. 2 Dendrogram of SmaI PFGE patterns for C. jejuni isolates from diarrhea patients.

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