Tissue Eng Regen Med.  2019 Apr;16(2):119-129. 10.1007/s13770-018-0149-3.

Efficient Production of Murine Uterine Damage Model

Affiliations
  • 1Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea. jyhsyk@snu.ac.kr
  • 2Department of Obstetrics and Gynecology, Korea University Guro Hospital, 148 Gurodong-ro, Guro-gu, Seoul 08308, Republic of Korea.
  • 3Department of Obstetrics and Gynecology, Jeju National University School of Medicine, 15 Aran 13-gil, Jeju-si, Jeju-do 63241, Republic of Korea.

Abstract

BACKGROUND
Thin or damaged endometrium causes uterine factor-derived infertility resulting in a failure of embryonic implantation. Regeneration of endometrium is a major issue in gynecology and reproductive medicine. Various types of cells and scaffolds were studied to establish an effective therapeutic strategy. For this type of investigations, production of optimal animal models is indispensable. In this study, we tried to establish various murine uterine damage models and compared their features.
METHODS
Three to ten-week-old C57BL/6 female mice were anesthetized using isoflurane. Chemical and mechanical methods using ethanol (EtOH) at 70 or 100% and copper scraper were compared to determine the most efficient condition. Damage of uterine tissue was induced either by vaginal or dorsal surgical approach. After 7-10 days, gross and microscopic morphology, safety and efficiency were compared among the groups.
RESULTS
Both chemical and mechanical methods resulted in thinner endometrium and reduced number of glands. Gross morphology assessment revealed that the damaged regions of uteri showed various shapes including shrinkage or cystic dilatation of uterine horns. The duration of anesthesia significantly affected recovery after procedure. Uterine damage was most effectively induced by dorsal approach using 100% EtOH treatment compared to mechanical methods.
CONCLUSION
Taken together, murine uterine damage models were most successfully established by chemical treatment. This production protocols could be applied further to larger animals such as non-human primate.

Keyword

Uterine damage; Endometrium thickness; Murine model; Embryonic implantation

MeSH Terms

Anesthesia
Animals
Copper
Dilatation
Endometrium
Ethanol
Female
Gynecology
Horns
Humans
Infertility
Isoflurane
Mice
Models, Animal
Primates
Regeneration
Reproductive Medicine
Uterus
Copper
Ethanol
Isoflurane
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