J Lipid Atheroscler.  2019 May;8(1):50-57. 10.12997/jla.2019.8.1.50.

Analysis of Circular RNAs in the Coronary Arteries of Patients with Kawasaki Disease

• Kim YK

• KMID: 2447756
• DOI: http://doi.org/10.12997/jla.2019.8.1.50

Keyword

Kawasaki disease; Coronary artery; RNA, circular; microRNAs

MeSH Terms

Coronary Vessels*
Humans
MicroRNAs
Mucocutaneous Lymph Node Syndrome*
RNA*
MicroRNAs
RNA

Figure

• 

  Fig. 1 Identification of circRNAs expressed in coronary artery tissue from control and Kawasaki disease groups. (A) The procedure used to identify circRNAs in the control and Kawasaki disease samples. Based on data from the GSE64486 dataset, the expression level of circRNAs in each group was calculated using the DCC algorithm and differentially expressed circRNAs were selected for further study.1316 (B) Average expression counts of highly expressed circRNAs. circRNAs with normalized average counts greater than 10 are shown. (C) The number of circRNAs produced from each gene was calculated. circRNA, circular RNA.

• 

  Fig. 2 Selected circRNAs with probable roles in Kawasaki disease. Among the circRNAs listed in Supplementary Table 2, only circRNAs that showed differential expression between control and untreated Kawasaki disease groups (p<0.05), and between untreated and treated Kawasaki disease groups (p<0.05), but no significant difference between the control and treated Kawasaki disease groups (p>0.05), were selected. p values were calculated by a t-test between samples from 2 groups, respectively. circRNA, circular RNA. *p<0.05; †p<0.01.

• 

  Fig. 3 Possible working mechanism of circRNAs as miRNA suppressors. (A) Analysis of circRNAs as miRNA suppressors. Among the increased miRNAs in patients with Kawasaki disease, miRNAs with predicted binding sites in circRNA sequences were selected using TargetScan.19 (B-C) The miRNA binding sites in circRNAs are depicted for (B) circWHAMMP1 and (C) circZNF124. (D) Base pairing between the predicted miRNA binding sites and circRNAs is shown. The nucleotide position of the back-splice junction depicted in (B, C) was set as zero, and the relative position of the nucleotide sequences was calculated. circRNA, circular RNA; miRNA, microRNA.

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