Korean J Physiol Pharmacol.  2019 Jan;23(1):21-28. 10.4196/kjpp.2019.23.1.21.

Swertiamarin ameliorates carbon tetrachloride-induced hepatic apoptosis via blocking the PI3K/Akt pathway in rats

• Zhang Q
• Chen K
• Wu T
• Song H

• KMID: 2443612
• DOI: http://doi.org/10.4196/kjpp.2019.23.1.21

Abstract

Swertiamarin (STM) is an iridoid compound that is present in the Gentianaceae swertia genus. Here we investigated antiapoptotic effects of STM on carbon tetrachloride (CCl₄)-induced liver injury and its possible mechanisms. Adult male Sprague Dawley rats were randomly divided into a control group, an STM 200 mg/kg group, a CCl₄ group, a CCl₄+STM 100 mg/kg group, and a CCl₄+STM 200 mg/kg group. Rats in experimental groups were subcutaneously injected with 40% CCl₄ twice weekly for 8 weeks. STM (100 and 200 mg/kg per day) was orally given to experimental rats by gavage for 8 consecutive weeks. Hepatocyte apoptosis was determined by TUNEL assay and the expression levels of Bcl-2, Bax, and cleaved caspase-3 proteins were evaluated by western blot analysis. The expression of TGF-β1, collagen I, collagen III, CTGF and fibronectin mRNA were estimated by qRT-PCR. The results showed that STM significantly reduced the number of TUNEL-positive cells compared with the CCl₄ group. The levels of Bax and cleaved caspase-3 proteins, and TGF-β1, collagen I, collagen III, CTGF, and fibronectin mRNA were significantly reduced by STM compared with the CCl₄ group. In addition, STM markedly abrogated the repression of Bcl-2 by CCl₄. STM also attenuated the activation of the PI3K/Akt pathway in the liver. These results suggested that STM ameliorated CCl₄-induced hepatocyte apoptosis in rats.

Keyword

Akt; Carbon tetrachloride; Collagen; Hepatocyte apoptosis; Swertiamarin

MeSH Terms

Adult
Animals
Apoptosis*
Blotting, Western
Carbon Tetrachloride
Carbon*
Caspase 3
Collagen
Fibronectins
Gentianaceae
Hepatocytes
Humans
In Situ Nick-End Labeling
Liver
Male
Rats*
Rats, Sprague-Dawley
Repression, Psychology
RNA, Messenger
Swertia
Carbon
Carbon Tetrachloride
Caspase 3
Collagen
Fibronectins
RNA, Messenger