Nat Prod Sci.  2019 Mar;25(1):11-15. 10.20307/nps.2019.25.1.11.

Chemical Constituents of Bark of Beilschmiedia kunstleri Gamble with Anticancer, Anti-Streptococcus pyogenes, Anti-Bacillus cereus and Anti Plesiomonas shigelloides Activities

Affiliations
  • 1Faculty of Science, Qom University of Technology, Qom, Iran. a_mollataghi2@yahoo.com
  • 2Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.

Abstract

Lauraceae is a family medicinal plant whose tubers possesses antimicrobial, and cytotoxic, such as antiparasitic and anti-inflammatory special effects and has been used for the medicine in the cure of hepatitis and rheumatism. The antimicrobial activities of bioactive compounds including one neolignan; kunstlerone (1) and two alkaloids include isocaryachine (2) and noratherosperminine (3) as well as crude hexane, methanol and dichloromethane extracts were evaluated. Additionally, the effect of compounds 1, 2 and 3 were evaluated on A549, PC-3, A375, HT-29 and WRL-68 cell lines. In conclusion, kunstlerone 1 showed moderate cytotoxicity against various cancer cell lines such as A549, PC-3, A375, HT-29 and WRL-68, respectively with ECâ‚…â‚€ values of 28.02, 26.78, 33.78, 33.65 and 16.46 µg/mL. The crude methanol extract showed antigrowth activity against S. pyogenes II and B. cereus, with MICs of 256 µg/mL. The compounds kunstlerone (1), isocaryachine (2) and noratherosperminine (3) showed complete inhibition against P. shigelloides, with MIC ≤60 µg/mL compare to ampicillin, as a positive control, which showed antigrowth activity against P. shigelloides at MIC 10 µg/mL.

Keyword

Beilschmiedia; lauraceae; alkaloid; kunstlerone; isocaryachine; Antimicrobial; noratherosperminine

MeSH Terms

Alkaloids
Ampicillin
Cell Line
Hepatitis
Humans
Lauraceae
Methanol
Methylene Chloride
Plants, Medicinal
Plesiomonas*
Rheumatic Diseases
Alkaloids
Ampicillin
Methanol
Methylene Chloride

Figure

  • Fig. 1 Chemical structurs of bark constituents (1 – 3).

  • Fig. 2 Dose-response curves (using GraphPad Prism) tested with kunstlerone (1), isocaryachine (2), noratherosperminine (3) and Doxorubicin (positive control) in the MTT assays towards A) A375, B) A549, C), HT-29, D) PC-3 and E) WRL-68.


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