Lab Med Online.  2019 Apr;9(2):77-83. 10.3343/lmo.2019.9.2.77.

Comparison between Medion Vacuon Tube and BD Vacutainer Tube for Clinical Laboratory Practice

Affiliations
  • 1Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea. juwon76@yonsei.ac.kr, cello425@yonsei.ac.kr

Abstract

BACKGROUND
Most of the blood-test samples are collected and carried in vacuum tubes. We have compared a newly developed vacuum tube, "˜Vacuon' (Medion, Korea) and "˜BD Vacutainer®' (BD, USA) in three common clinical assays, i.e., hematological, chemical, and immunological tests.
METHODS
A sum of 60 healthy volunteers were recruited in our study and their peripheral blood samples were collected in the tubes of the two brands. EDTA-tube samples were evaluated using 25 hematological tests. Serum separating tube samples were analyzed for 24 chemical parameters and the 3 thyroid hormones. The results were statistically analyzed using the paired t-test and Bland-Altman plot. In addition, the assay outcomes at t=0 hr were compared with those at t=24±2 hr for each of the tubes.
RESULTS
The assay results of 22 hematological parameters, 24 chemical parameters, and 3 thyroid hormones had a statistically significant correlation between the 2 brands of vacuum tubes (t=0 hr). Two hematological parameters (mean corpuscular hemoglobin concentration [MCHC] and cell hemoglobin concentration mean [CHCM]) showed higher mean values, while a hematological parameter (lobularity index [LI]) showed lower mean values in Vacuon than BD Vacutainer (t=0 hr). The results after 24 hr showed similarity between the 2 brands, with some inconsistent results in BD Vacutainer (Mean platelet volume [MPV], plateletcrit [Pct], eosinophil, calcium, and triiodothyronine) and Vacuon (MPV, hemoglobin distribution width [HDW], CHCM, Pct, eosinophil, and calcium).
CONCLUSIONS
BD Vacutainer and Vacuon tube showed good statistical concordance rate with some exceptions in the hematological parameters (MCHC, CHCM, and LI).

Keyword

Comparison; Vacuum tube; Concordance; Parameters

MeSH Terms

Blood Platelets
Calcium
Eosinophils
Healthy Volunteers
Hematologic Tests
Immunologic Tests
Thyroid Hormones
Vacuum
Calcium
Thyroid Hormones

Figure

  • Fig. 1. Bland-Altman plot of the difference of results between BD Vacutainer and Vacuon (A) mean corpuscular hemoglobin concentration (MCHC), (B) cell hemoglobin concentration mean (CHCM), (C) lobularity index (LI). Abbreviations: ULOA, upper limit of agreement; LLOA, lower limit of agreement.


Reference

References

1. Narayanan S. The preanalytic phase. An important component of laboratory medicine. Am J Clin Pathol. 2000; 113:429–52.
2. Lippi G, Guidi GC, Mattiuzzi C, Plebani M. Preanalytical variability: the dark side of the moon in laboratory testing. Clin Chem Lab Med. 2006; 44:358–65.
Article
3. Oh SH and Ki CS. Comparison of two new plastic tubes (Sekisui INSE-PACK and Green Cross Green Vac-Tube) with BD Vacutainer tubes for 49 analytes. Korean J Lab Med. 2007; 27:69–75.
4. Landis JR and Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977; 33:159–74.
Article
5. Kim JY, Nam DH, Kim SH, Yang JH, Yoon SY, Lim CS, et al. Evaluation of Green Vac-Tube in clinical laboratory tests. J Lab Med Qual Assur. 2008; 30:307–14.
6. Medicare, Medicaid and CLIA programs; regulations implementing the Clinical Laboratory Improvement Amendments of 1988 (CLIA)-HCFA. Final rule with comment period. Fed Regist. 1992; 57:7002–186.
7. Hill VL, Simpson VZ, Higgins JM, Hu Z, Stevens RA, Metcalf JA, et al. Evaluation of the performance of the Sysmex XT-2000i hematology analyzer with whole bloods stored at room temperature. Lab Med. 2009; 40:709–18.
8. Pinter E, Laszlo K, Schuszler I, Konderak J. The stability of quantitative blood count parameters using the ADVIA 2120i hematology analyzer. Pract Lab Med. 2015; 4:16–21.
9. Won EJ, Shin MG, Jang MJ, Cho D, Kee SJ, Kim SH, et al. Comparison between V-tubes and BD Vacutainer tubes for use in laboratory tests. Lab Med Online. 2013; 3:145–54.
Article
10. Ismail Y, Ismail AA, Ismail AA. Erroneous laboratory results: what clinicians need to know. Clin Med (Lond). 2007; 7:357–61.
Article
Full Text Links
  • LMO
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr