Korean J Parasitol.  2019 Feb;57(1):49-53. 10.3347/kjp.2019.57.1.49.

Effect of Temperature on the Killing of Opisthorchis viverrini Eggs In Vitro

Affiliations
  • 1Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. bthida@kku.ac.th
  • 2Neglected, zoonosis, and vector-borne disease group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
  • 3Liver Fluke and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.
  • 4Department of Public Health, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000, Thailand.
  • 5Department of Anesthesiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
  • 6Department of Community Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

Abstract

Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis associated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Mature eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80°C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were observed under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80°C groups were all killed after over 10 min heated. Majority of eggs in 60°C (10, 15, and 30 min heated), 70 and 80°C (5 min heated) groups were inactivated. However in 50°C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80°C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a potential method for controlling egg contamination in sewage.

Keyword

Opisthorchis viverrini; egg; heating; temperature; treatment

MeSH Terms

Adult
Animals
Asia, Southeastern
Cholangiocarcinoma
Cricetinae
Eggs*
Fasciola hepatica
Heating
Homicide*
Hot Temperature
Humans
In Vitro Techniques*
Methods
Opisthorchiasis
Opisthorchis*
Ovum*
Prevalence
Propidium
Sewage
Uterus
Propidium
Sewage
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