Korean J Parasitol.  2019 Feb;57(1):27-31. 10.3347/kjp.2019.57.1.27.

Comparison of Two PCR Assays for Trichomonas vaginalis

Affiliations
  • 1Department of Internal Medicine, Hallym University Hangang Sacred Heart Hospital, Seoul 07247, Korea.
  • 2Department of Environmental Biology and Medical Parasitology, Hanyang University Graduate School of Biomedical Science and Engineering, Seoul 04763, Korea. jsryu@hanyang.ac.kr
  • 3Department of Biomedical Science, Hanyang University Graduate School of Biomedical Science and Engineering, Seoul 04763, Korea.
  • 4Department of Urology, Hanyang University College of Medicine, Seoul 04763, Korea.
  • 5Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea.

Abstract

PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

Keyword

Trichomonas vaginalis; PCR; DNA isolation
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