Ann Dermatol.  2019 Apr;31(2):186-195. 10.5021/ad.2019.31.2.186.

Adiponectin Attenuates the Inflammation in Atopic Dermatitis-Like Reconstructed Human Epidermis

Affiliations
  • 1Department of Dermatology, Dankook University College of Medicine, Cheonan, Korea. zamoo97@naver.com
  • 2Department of Dermatology, Chungnam National University College of Medicine, Daejeon, Korea.
  • 3Department of Dermatology, Chung-Ang University Hospital, Seoul, Korea.

Abstract

BACKGROUND
Atopic dermatitis (AD) is a chronic disorder, with a vicious cycle of repetitive inflammation and deterioration of the epidermal barrier function. Adiponectin, an adipokine, has anti-inflammatory effects on various metabolic and inflammatory disorders. Recently, its level was found to be reduced in serum and tissue samples from AD patients.
OBJECTIVE
We aimed to investigate the effects of adiponectin on epidermal inflammation and barrier structures in AD skin.
METHODS
A three-dimensional in vitro epidermal equivalent model mimicking AD was obtained by adding an inflammatory substance cocktail to normal human epidermal equivalents (HEEs). The expression of epidermal differentiation markers, primary inflammatory mediators, and lipid biosynthetic enzymes was compared between adiponectintreated AD-HEEs, untreated control AD-HEEs, and normal HEEs.
RESULTS
Adiponectin co-treatment 1) inhibited the increase in mRNA expression of major inflammatory mediators (carbonic anhydrase II, neuron-specific NEL-like protein 2, thymic stromal lymphopoietin, interleukin-8, tumor necrosis factor-alpha, and human beta-defensin-2) from keratinocytes in AD-inflammatory HEEs, 2) enhanced the expression of lipid biosynthetic enzymes (fatty acid synthase, HMG CoA reductase, and serine-palmitoyl transferase), and 3) promoted the expression of differentiation factors, especially filaggrin. We also found that the expression of adiponectin receptor-1 and -2 decreased in the epidermis of chronic AD lesion.
CONCLUSION
Activation of the adiponectin pathway is expected to enhance epidermal differentiation and barrier function as well as attenuate inflammatory response to AD as a therapeutic approach.

Keyword

Adiponectin; Atopic dermatitis; Epidermal barrier; Keratinocyte; Lipid synthesis

MeSH Terms

Adipokines
Adiponectin*
Antigens, Differentiation
Dermatitis, Atopic
Epidermis*
Humans*
Hydroxymethylglutaryl CoA Reductases
In Vitro Techniques
Inflammation*
Interleukin-8
Keratinocytes
RNA, Messenger
Skin
Tumor Necrosis Factor-alpha
Adipokines
Adiponectin
Antigens, Differentiation
Hydroxymethylglutaryl CoA Reductases
Interleukin-8
RNA, Messenger
Tumor Necrosis Factor-alpha
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