Korean J Vet Res.
2018 Mar;58(1):51-55. 10.14405/kjvr.2018.58.1.51.
Molecular identification of coagulase-negative staphylococci by rpoB sequence typing
- Affiliations
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- 1Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. kwonhj01@snu.ac.kr
- 2Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
- 3Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
- 4Farm Animal Clinical Training and Research Center, Institutes of GreenBio Science Technology, Pyeongchang 25354, Korea.
- KMID: 2437678
- DOI: http://doi.org/10.14405/kjvr.2018.58.1.51
Abstract
- Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.
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