Int J Oral Biol.  2018 Jun;43(2):77-82. 10.11620/IJOB.2018.43.2.077.

Differentiation of CD31-Positive Vascular Endothelial Cells from Organoid Culture of Dental Pulp Stem Cells

Affiliations
  • 1Department of Oral Biochemistry and Molecular Biology, Pusan National University School of Dentistry, Yangsan 50612, Gyeongsangnam-do, Republic of Korea. ilho.jang@pusan.ac.kr
  • 2Research Institute of Translational Dental Sciences, Pusan National University, Yangsan 50612, Gyeongsangnam-do, Republic of Korea.
  • 3DGIST, Daegu 42988, Republic of Korea.

Abstract

The mesenchymal stem cells (MSCs) that reside in dental tissues hold a great potential for future applications in regenerative dentistry. In this study, we used human dental pulp cells, isolated from the molars (DPCs), in order to establish the organoid culture. DPCs were established after growing pulp cells in an MSC expansion media (MSC-EM). DPCs were subjected to organoid growth media (OGM) in comparison with human dental pulp stem cells (DPSCs). Inside the extracellular matrix in the OGM, the DPCs and DPSCs readily formed vessel-like structures, which were not observed in the MSC-EM. Immunocytochemistry analysis and flow cytometry analysis showed the elevated expression of CD31 in the DPCs and DPSCs cultured in the OGM. These results suggest endothelial cell-prone differentiation of the DPCs and DPSCs in organoid culture condition.

Keyword

dental pulp stem cell; organoid culture; endothelial cell; CD31

MeSH Terms

Dental Pulp*
Dentistry
Endothelial Cells*
Extracellular Matrix
Flow Cytometry
Humans
Immunohistochemistry
Mesenchymal Stromal Cells
Molar
Organoids*
Stem Cells*
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