Int J Oral Biol.  2018 Sep;43(3):141-146. 10.11620/IJOB.2018.43.3.141.

Xylitol Mitigate Neutrophil Inflammatory Response Against Porphyromonas gingivalis Infection

Affiliations
  • 1Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea. jchung@pusan.ac.kr
  • 2Department of Hematology-Oncology, Dongnam Institute of Radiological and Medical Sciences, Pusan 46033, Republic of Korea.

Abstract

Periodontitis is generally a chronic disorder characterized by breakdown of tooth-supporting tissues, producing dentition loss. Porphyromonas gingivalis (P. gingivalis), a Gram-negative anaerobic rod, is one of the major pathogens associated with periodontitis. Neutrophils are first line defense cells in the oral cavity that play a significant role in inflammatory response. Xylitol is a known anti-caries agent and has anti-inflammatory effects. In this study, we conducted experiments to evaluate anti-inflammatory effects of xylitol on P. gingivalis infected neutrophils for possible usage in prevention and treatment of periodontal infections. P. gingivalis was intraperitoneally injected and peritoneal lavage was collected for cytokine determination. For in vitro study, neutrophils were collected from mouse peritoneal cells after zymosan injection or bone marrow cells. Neutrophils were stimulated with live P. gingivalis and ELISA was used to determine the effect of xylitol on P. gingivalis induced cytokine production. IL-1β, IL-6, TNF-α concentration and neutrophil population in the peritoneal lavage was increased in P. gingivalis-infected mouse. Peritoneal cells infected with live P. gingivalis revealed significantly increased production of IL-1β, IL-6 and TNF-α at multiplicity of infection of 10. Neutrophils from bone marrow and peritoneal lavage revealed increased production of IL-1β, IL-6 and TNF-α. Xylitol significantly mitigated P. gingivalis induced cytokine production in neutrophils. Findings indicate that xylitol is an anti-inflammatory agent in neutrophils infected with live P. gingivalis, that suggests its use in periodontitis management.

Keyword

P. gingivalis; neutrophil; xylitol; inflammation; periodontitis

MeSH Terms

Animals
Bone Marrow
Bone Marrow Cells
Dentition
Enzyme-Linked Immunosorbent Assay
In Vitro Techniques
Inflammation
Interleukin-6
Mice
Mouth
Neutrophils*
Periodontitis
Peritoneal Lavage
Porphyromonas gingivalis*
Porphyromonas*
Xylitol*
Zymosan
Interleukin-6
Xylitol
Zymosan
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