J Obes Metab Syndr.  2018 Dec;27(4):248-253. 10.7570/jomes.2018.27.4.248.

Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1

Affiliations
  • 1Department of Biochemistry, Keimyung University School of Medicine, Daegu, Korea.
  • 2Department of Anatomy and Cell Biology, Hanyang University College of Medicine, Seoul, Korea.
  • 3Department of Surgery, Hanyang University College of Medicine, Seoul, Korea. missurgeon@hanyang.ac.kr

Abstract

BACKGROUND
Sirtuins mediate metabolic responses to nutrient availability and slow aging and accompanying decline in health. This study was designed to assess the expressions of sirtuin1 (SIRT1) and sirtuin3 (SIRT3) in the liver and hypothalamus after duodenal-jejunal bypass (DJB) surgery in rats.
METHODS
A total of 38 rats were randomly assigned to either sham group (n=8) or DJB group (n=30). DJB group was again divided into three groups according to the elapsed time after surgery (10 weeks, DJB10; 16 week, DJB16; 28 week, DJB28). The mRNA and protein expressions of SIRT1 and SIRT3 in the liver and hypothalamus were measured by reverse transcription polymerase chain reaction, Western blot, and immunohistochemistry analyses. NAD⁺/NADH ratio was also measured.
RESULTS
We found increased mRNA and protein expression levels of SIRT1 in the liver of DJB16 and DJB28 groups compared with those of sham group. The mRNA and protein expressions of SIRT3 in the liver of DJB group increased proportionally to the elapsed time after DJB surgery. The mRNA expression levels of SIRT1 in the hypothalamus increased in DJB16 and DJB28 groups and protein expression levels of SIRT1 in the hypothalamus increased in DJB10, DBJ16, and DJB28 groups compared with sham group. We observed that mRNA and protein levels of SIRT3 in the hypothalamus of DJB group were not changed.
CONCLUSION
This study proves that DJB increases SIRT1 and SIRT3 expressions in the liver and SIRT1 expression in the hypothalamus. These results suggest the possibility of sirtuins being involved in bypass surgery-induced metabolic changes.

Keyword

Duodenojejunal bypass; Life span; Sirtuin1; Sirtuin3

MeSH Terms

Aging
Animals
Blotting, Western
Hypothalamus
Immunohistochemistry
Liver
Polymerase Chain Reaction
Rats
Reverse Transcription
RNA, Messenger
Sirtuins
RNA, Messenger
Sirtuins
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