Nat Prod Sci.  2018 Dec;24(4):293-297. 10.20307/nps.2018.24.4.293.

Effect of Sasa quelpaertensis Nakai Extracts and its Constituent p-coumaric Acid on the Apoptosis of Human Cancer Cell Lines

• Jang MG
• Ko HC
• Kim SJ

• KMID: 2432426
• DOI: http://doi.org/10.20307/nps.2018.24.4.293

Abstract

Sasa quelpaertensis Nakai leaves contain a mixture of polysaccharides, amino acids, and polyphenols, which mediate various biological activities. For efficient utilization of its leaf, we reported the preparation procedure for phytochemical-rich extract (PRE) using the leaf residue, which was by-product of hot water extraction. This study was undertaken to evaluate the effects of PRE and its major constituent, p-coumaric acid,on the growth of several human cancer cell lines (MKN-74, MKN-45, SNU-1, SNU-16, and HL-60). The ethyl acetate fraction of PRE and p-coumaric acid significantly inhibited the proliferation of MKN-74 and HL-60 cells, respectively, and induced cell apoptosis, down-regulated Bcl-2 and poly (ADP-ribose) polymerase levels, and up-regulated those of Bax and caspase-3. These results show the potential utility of S. quelpaertensis Nakai leaves in cancer prevention.

Keyword

Apoptosis; p-coumaric acid; phytochemicals; Sasa quelpaertensis Nakai

MeSH Terms

Amino Acids
Apoptosis*
Caspase 3
Cell Line*
HL-60 Cells
Humans*
Phytochemicals
Polyphenols
Polysaccharides
Sasa*
Water
Amino Acids
Caspase 3
Polyphenols
Polysaccharides
Water

Figure

• 

  Fig. 1 Growth-inhibiting effects of phytochemical-rich extract (PRE) fraction (A) and p-coumaric acid (B) on the several human cancer cell lines. Data are means ± standard deviation (SD) of three independent experiments. ***p < 0.001, **p < 0.01, and *p < 0.05 compared to the untreated group.

• 

  Fig. 2 Apoptosis inducing effects of ethyl acetate fraction of PRE (EPRE) (A) and p-coumaric acid (B) in HL-60 cells. Data (means ± SD of triplicate determinations) are representative of at least three independent experiments. ***p < 0.001 compared to the untreated group. (A) PRE, (B) p-coumaric acid.

• 

  Fig. 3 Effects of ethyl acetate fraction of PRE (EPRE) and p-coumaric acid on the expression of apoptosis-related proteins in MKN-74 cells (A, C) and HL-60 cells (B, D).

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