Nat Prod Sci.  2018 Dec;24(4):247-252. 10.20307/nps.2018.24.4.247.

Comparison of Biological Activities of Korean Halophytes

• Lee JM
• Yim MJ
• Lee DS
• Lee MS
• Park YG
• Jeon JH
• Choi G

• KMID: 2432419
• DOI: http://doi.org/10.20307/nps.2018.24.4.247

Abstract

Halophytes are expected to possess abundant secondary metabolites and various biological activities because of habitat in extreme environments. In this study, we collected 14 halophytes (Asparagus oligoclonos, Calystegia soldanella, Carex pumila, Chenopodium glaucum, Elymus mollis, Glehnia littoralis, Limonium tetragonum, Messerschmidia sibirica, Rosa rugosa, Salsola komarovii, Spergularia marina, Suaeda glauca, Suaeda maritima, and Vitex rotundifolia) native to Korea and compared their total polyphenol contents, antioxidant and anti-inflammatory activities. The total polyphenol contents of R. rugosa (27.28%) and L. tetragonum (13.17%) were significantly higher than those of the other 12 halophytes and L. tetragonum, R. rugosa, and M. sibirica showed significantly greater antioxidant activities than the other 11 halophytes, as determined by DPPH (2,2-diphenyl-1-picrylhydrazyl). A. oligoclonos, E. mollis, and C. pumila showed significantly greater anti-inflammatory activities than the other 11, as determined by NO (Nitric oxide) and PGEâ‚‚ (Prostaglandin Eâ‚‚) levels. In contrast, these three extracts had normal and low total polyphenol contents among the 14 halophytes. Consequently, the total polyphenol content in the 14 studied halophytes appeared to be related to antioxidant, but not anti-inflammatory activity levels.

Keyword

Antioxidant; Anti-inflammatory; Halophyte plants; Polyphenol; NO; PGEâ‚‚

MeSH Terms

Apiaceae
Calystegia
Caryophyllaceae
Chenopodiaceae
Chenopodium
Ecosystem
Elymus
Korea
Plumbaginaceae
Rosa
Salsola
Salt-Tolerant Plants*
Vitex

Figure

• 

  Fig. 1 DPPH radical scavenging activity of 14 halophyte extracts expressed as half maximal inhibitory concentration (IC50). All the data are shown as mean ± SD from at least three independent experiments performed in triplicate.

• 

  Fig. 2 Cell viability was evaluated using a CCK-8 assay after treatment with various concentrations of halophytes (10~500 µg/mL). The data are represented as % of the untreated control. All the data are shown as mean ± SD from at least three independent experiments performed in triplicate.

• 

  Fig. 3 Inhibition of NO production after the treatment of lipopolysaccharide-stimulated RAW 264.7 cells with extracts from 14 halophytes. The experiment was performed in triplicate and data are represented as mean ± SD. *p<0.05; **p<0.01; ***p<0.001.

• 

  Fig. 4 Inhibition of PGE2 release in lipopolysaccharide-stimulated RAW 264.7 cells after treatment with extracts from 14 halophytes. The experiment was performed in triplicate and data are represented as mean ± SD. *p<0.05; **p<0.01; ***p<0.001.

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