Lab Med Online.  2019 Jan;9(1):17-21. 10.3343/lmo.2019.9.1.17.

Evaluation of the Real-Q RV Detection Kit for the Identification of Viruses That Result in Respiratory Infections

• Lee E
• Shin S
• Kim M
• Lee YK
• Kang HJ
• Kim HS
• Kim JS
• Song W
• Kim HS

• KMID: 2430205
• DOI: http://doi.org/10.3343/lmo.2019.9.1.17

Abstract

Viral respiratory infections are one of the most common infections worldwide. It is important to detect the virus early and precisely. In this study, we evaluated the limit of detection (LoD) and usefulness of the Real-Q RV Detection kit (BioSewoom, Seoul, Korea). We measured the LoD of the Real-Q RV Detection kit using 10 strains of standard viruses. We then compared the detection results by the Allplex Respiratory Panel Assay kit (Seegene, Seoul, Korea) using 123 clinical specimens. The discrepant results were confirmed by sequencing. Among the 10 standard viruses, the LoD of human rhinovirus (HRV) was the lowest and that of parainfluenza virus 2 and 3 was relatively high as detected by Real-Q RV Detection kit. Agreements of the two kits ranged from 95.9% to 100%. Three specimens detected negative by the Allplex Respiratory Panel kit were detected as adenovirus (AdV) by the Real-Q RV Detection kit and were confirmed by sequencing. Similarly, a specimen detected negative by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. A specimen detected as human enterovirus by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. Real-Q RV Detection kit showed good diagnostic performance and can be useful for detecting major viruses that cause respiratory infections.

Keyword

Respiratory virus; Multiplex real-time PCR; Real-Q RV Detection kit

MeSH Terms

Adenoviridae
Enterovirus
Humans
Limit of Detection
Paramyxoviridae Infections
Respiratory Tract Infections*
Rhinovirus
Seoul

Reference

1.Thompson WW., Shay DK., Weintraub E., Brammer L., Cox N., Anderson LJ, et al. Mortality associated with infuenza and respiratory syncytial virus in the United States. JAMA. 2003. 289:179–86.

2.Lee KH., Gordon A., Foxman B. The role of respiratory viruses in the etiology of bacterial pneumonia: an ecological perspective. Evol Med Public Health. 2016. 95–109.

3.Garbino J., Gerbase MW., Wunderli W., Deffernez C., Thomas Y., Rochat T, et al. Lower respiratory viral illnesses: improved diagnosis by molecular methods and clinical impact. Am J Respir Crit Care Med. 2004. 170:1197–203.

4.Charles PG. Early diagnosis of lower respiratory tract infections (point-of-care tests). Curr Opin Pulm Med. 2008. 14:176–82.

5.Vallières E., Renaud C. Clinical and economical impact of multiplex respiratory virus assays. Diag Microbiol Infect Dis. 2013. 76:255–61.

6.Mahony JB., Petrich A., Smieja M. Molecular diagnosis of respiratory virus infections. Crit Rev Clin Lab Sci. 2011. 48:217–49.

7.Kim S., Eom KW., Cho CR., Um TH. Comparison of the ability of multiplex and singleplex PCR to detect human respiratory viruses. Lab Med Online. 2016. 6:240–5.

8.Rheem I., Park J., Kim TH., Kim JW. Evaluation of a multiplex real-time PCR assay for the detection of respiratory viruses in clinical specimens. Ann Lab Med. 2012. 32:399–406.

9.Huh HJ., Kim JY., Kwon HJ., Yun SA., Lee MK., Lee NY, et al. Performance evaluation of Allplex Respiratory Panels 1, 2, and 3 for detection of respiratory viruses and infuenza A virus subtypes. J Clin Microbiol. 2017. 55:479–84.

10.Butt S., Maceira VP., McCallen ME., Stellrecht KA. Comparison of three commercial RT-PCR systems for the detection of respiratory viruses. J Clin Virol. 2014. 61:406–10.

11.Krunic N., Yager TD., Himsworth D., Merante F., Yaghoubian S., Janec-zko R. xTAG RVP assay: analytical and clinical performance. J Clin Virol. 2007. 40:S39–46.

12.Gharabaghi F., Hawan A., Drews SJ., Richardson SE. Evaluation of multiple commercial molecular and conventional diagnostic assays for the detection of respiratory viruses in children. Clin Microbiol Infect. 2011. 17:1900–6.

13.Jo YA., Kim HS., Lee YK., Kim JS., Song W., Kang HJ, et al. Interpretation of weak-positive bands in a multiplex PCR using Seeplex RV12 ACE detection kit. J Lab Med Qual Assur. 2011. 33:89–93.

14.Le SY., Chen JH., Sonenberg N., Maizel JV. Conserved tertiary structure elements in the 5′untranslated region of human enteroviruses and rhinoviruses. Virology. 1992. 191:858–66.

15.Dabisch-Ruthe M., Vollmer T., Adams O., Knabbe C., Dreier J. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infect Dis. 2012. 12:163.

16.Ko DH., Kim HS., Hyun J., Kim HS., Kim JS., Park KU, et al. Comparison of the Luminex xTAG Respiratory Viral Panel Fast v2 Assay with Any-plex II RV16 detection Kit and AdvanSure RV Real-Time RT-PCR Assay for the detection of respiratory viruses. Ann Lab Med. 2017. 37:408–14.