Ann Lab Med.  2018 Nov;38(6):610-612. 10.3343/alm.2018.38.6.610.

Utility of 16S rRNA PCR in the Synovial Fluid for the Diagnosis of Prosthetic Joint Infection

• Sebastian
• Malhotra R
• Sreenivas
• Kapil A
• Chaudhry R
• Dhawan B

• KMID: 2429130
• DOI: http://doi.org/10.3343/alm.2018.38.6.610

Abstract

No abstract available.

MeSH Terms

Diagnosis*
Joints*
Polymerase Chain Reaction*
Synovial Fluid*

Reference

1. Parvizi J, Zmistowski B, Berbari EF, Bauer TW, Springer BD, Della Valle CJ, et al. New definition for periprosthetic joint infection: from the Workgroup of the Musculoskeletal Infection Society. Clin Orthop Relat Res. 2011; 469:2992–2994. PMID: 21938532.

2. Gallo J, Kolar M, Dendis M, Loveckova Y, Sauer P, Zapletalova J, et al. Culture and PCR analysis of joint fluid in the diagnosis of prosthetic joint infection. New Microbiol. 2008; 31:97–104. PMID: 18437847.

3. Melendez DP, Greenwood-Quaintance KE, Berbari EF, Osmon DR, Mandrekar JN, Hanssen AD, et al. Evaluation of a genus-and group-specific rapid PCR assay panel on synovial fluid for diagnosis of prosthetic knee infection. J Clin Microbiol. 2016; 54:120–126. PMID: 26537446.

4. Rak M, KavčIč M, Trebše R, CőR A. Detection of bacteria with molecular methods in prosthetic joint infection: sonication fluid better than periprosthetic tissue. Acta Orthop. 2016; 87:339–345. PMID: 27123818.

5. Panousis K, Grigoris P, Butcher I, Rana B, Reilly JH, Hamblen DL. Poor predictive value of broad-range PCR for the detection of arthroplasty infection in 92 cases. Acta Orthop. 2005; 76:341–346. PMID: 16156461.

6. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991; 173:697–703. PMID: 1987160.

7. Bémer P, Plouzeau C, Tande D, Léger J, Giraudeau B, Valentin AS, et al. Evaluation of 16S rRNA gene PCR sensitivity and specificity for diagnosis of prosthetic joint infection: a prospective multicenter cross-sectional study. J Clin Microbiol. 2014; 52:3583–3589. PMID: 25056331.

8. Haag H, Locher F, Nolte O. Molecular diagnosis of microbial aetiologies using SepsiTest in the daily routine of a diagnostic laboratory. Diagn Microbiol Infect Dis. 2013; 76:413–418. PMID: 23747029.

9. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Kaczmarski EB, Fox AJ. Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J Clin Microbiol. 2000; 38:1747–1752. PMID: 10790092.

10. Heininger A, Binder M, Ellinger A, Botzenhart K, Unertl K, Doring G. DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results. J Clin Microbiol. 2003; 41:1763–1765. PMID: 12682181.