Ann Dermatol.  2018 Dec;30(6):751-754. 10.5021/ad.2018.30.6.751.

Effects of Extracellular Calcium and Vitamin D on Cultured Human Sebocytes

Affiliations
  • 1Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
  • 2Department of Dermatology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, Korea. weonju@knu.ac.kr

Abstract

No abstract available.


MeSH Terms

Calcium*
Humans*
Vitamin D*
Vitamins*
Calcium
Vitamin D
Vitamins

Figure

  • Fig. 1 (A) Gene expression of biomarkers in cultured human sebocytes after treatment with calcium (0.25, 0.5, 1, and 1.2 mM). (B) Protein expression of biomarkers in cultured human sebocytes after treatment with calcium (0.25, 0.5, 0.75, 1, and 1.2 mM) using ELISA. (C) Protein expression of biomarkers in cultured human sebocytes after treatment with calcium (0.25, 0.5, 0.75, 1, and 1.2 mM) using western blot analysis. IL: interleukin, TNF: tumor necrosis factor, MC-1R: melanocortin-1 receptor.

  • Fig. 2 (A) Gene expression of biomarkers in cultured human sebocytes after treatment with vitamin D (10−6 M) and calcium (0.25 or 1.2 mM). (B) Protein expression of biomarkers in cultured human sebocytes after treatment with vitamin D (10−6 M) and calcium (0.25 or 1.2 mM) using ELISA. (C) Protein expression of biomarkers in cultured human sebocytes after treatment with vitamin D (10−6 M) and calcium (0.25 or 1.2 mM) using western blot analysis. IL: interleukin, TNF: tumor necrosis factor, MC-1R: melanocortin-1 receptor. *p<0.05.


Reference

1. Lesnik RH, Kligman LH, Kligman AM. Agents that cause enlargement of sebaceous glands in hairless mice. II. Ultraviolet radiation. Arch Dermatol Res. 1992; 284:106–108.
Article
2. Kurihara H, Sato T, Akimoto N, Ito A. Differentiated hamster sebocytes exhibit apoptosis-resistant phenotype by the augmentation of intracellular calcium level in vitro. Exp Dermatol. 2013; 22:57–59.
Article
3. Menon GK, Grayson S, Elias PM. Ionic calcium reservoirs in mammalian epidermis: ultrastructural localization by ioncapture cytochemistry. J Invest Dermatol. 1985; 84:508–512.
Article
4. Bikle DD, Ng D, Tu CL, Oda Y, Xie Z. Calcium- and vitamin D-regulated keratinocyte differentiation. Mol Cell Endocrinol. 2001; 177:161–171.
Article
5. Bikle DD. Vitamin D: an ancient hormone. Exp Dermatol. 2011; 20:7–13.
Article
6. Krämer C, Seltmann H, Seifert M, Tilgen W, Zouboulis CC, Reichrath J. Characterization of the vitamin D endocrine system in human sebocytes in vitro. J Steroid Biochem Mol Biol. 2009; 113:9–16.
Article
7. Zouboulis CC, Seltmann H, Abdel-Naser MB, Hossini AM, Menon GK, Kubba R. Effects of extracellular calcium and 1,25 dihydroxyvitamin D3 on sebaceous gland cells in vitro and in vivo. Acta Derm Venereol. 2017; 97:313–320.
Article
8. Whang SW, Lee SE, Kim JM, Kim HJ, Jeong SK, Zouboulis CC, et al. Effects of α-melanocyte-stimulating hormone on calcium concentration in SZ95 sebocytes. Exp Dermatol. 2011; 20:19–23.
Article
9. Martinsson H, Yhr M, Enerbäck C. Expression patterns of S100A7 (psoriasin) and S100A9 (calgranulin-B) in keratinocyte differentiation. Exp Dermatol. 2005; 14:161–168.
Article
10. Pernet I, Reymermier C, Guezennec A, Branka JE, Guesnet J, Perrier E, et al. Calcium triggers beta-defensin (hBD-2 and hBD-3) and chemokine macrophage inflammatory protein-3 alpha (MIP-3alpha/CCL20) expression in monolayers of activated human keratinocytes. Exp Dermatol. 2003; 12:755–760.
Article
Full Text Links
  • AD
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr