Immune Netw.  2018 Aug;18(4):e25. 10.4110/in.2018.18.e25.

Murine γδ T Cells Render B Cells Refractory to Commitment of IgA Isotype Switching

  • 1Department of Molecular Bioscience, College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
  • 2School of Life Sciences and BioImaging and Immune Synapse Research Center, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea.
  • 3Division of Biomedical Convergence, School of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
  • 4Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon 24341, Korea.
  • 5College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea.


γδ T cells are abundant in the gut mucosa and play an important role in adaptive immunity as well as innate immunity. Although γδ T cells are supposed to be associated with the enhancement of Ab production, the status of γδ T cells, particularly in the synthesis of IgA isotype, remains unclear. We compared Ig expression in T cell receptor delta chain deficient (TCRδ⁻/⁻) mice with wild-type mice. The amount of IgA in fecal pellets was substantially elevated in TCRδ⁻/⁻ mice. This was paralleled by an increase in surface IgA expression and total IgA production by Peyer's patches (PPs) and mesenteric lymph node (MLN) cells. Likewise, the TCRδ⁻/⁻ mice produced much higher levels of serum IgA isotype. Here, surface IgA expression and number of IgA secreting cells were also elevated in the culture of spleen and bone marrow (BM) B cells. Germ-line α transcript, an indicator of IgA class switch recombination, higher in PP and MLN B cells from TCRδ⁻/⁻ mice, while it was not seen in inactivated B cells. Nevertheless, the frequency of IgA+ B cells was much higher in the spleen from TCRδ⁻/⁻ mice. These results suggest that γδ T cells control the early phase of B cells, in order to prevent unnecessary IgA isotype switching. Furthermore, this regulatory role of γδ T cells had lasting effects on the long-lived IgA-producing plasma cells in the BM.


gamma delta; B-Lymphocytes; Immunoglobulin A; Immunoglobulin class switching

MeSH Terms

Adaptive Immunity
Bone Marrow
Immunity, Innate
Immunoglobulin A*
Immunoglobulin Class Switching*
Lymph Nodes
Mucous Membrane
Peyer's Patches
Plasma Cells
Receptors, Antigen, T-Cell, gamma-delta
Recombination, Genetic
Immunoglobulin A
Receptors, Antigen, T-Cell, gamma-delta
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